PMID- 26352330
OWN - NLM
STAT- MEDLINE
DCOM- 20160120
LR  - 20220408
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 30
IP  - 8
DP  - 2015 Aug
TI  - Is it possible folic acid reduce anorectal malformations ethylenethiourea induced 
      in rats?
PG  - 517-22
LID - S0102-86502015000800517 [pii]
LID - 10.1590/S0102-865020150080000001 [doi]
AB  - PURPOSE: To investigate the effect of folic acid (FA) in an experimental model of 
      anorectal malformations (ARMs) ethylenethiourea (ETU) induced. METHODS: Eight 
      female Wistar rats were divided randomly in two groups. Group A - ETU; Group B - 
      FA+ETU; Dams from group B received daily, since two weeks before pregnancy to the 
      end of pregnancy, FA (50mg/kg) by gavage. Dams from groups A and B, received 1% 
      ETU (125 mk/kg) by gavage on gestational day (GD) 11. Their fetuses were 
      harvested by cesarean section on GD21 and were examined looking for ARMs. The 
      thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium 
      (IE) were analyzed. p < 0.05*. RESULTS: One hundred and one embryos were 
      harvested. The number of embryos; number of ARMs; mean statistical % (+/- SD) were 
      determined to be, respectively: ETU - 49 [30;65% (+/- 24%)] versus FA+ETU - 52 
      [1;02% (+/- 3%)] (p = 0.025). AMRs were significantly lower in FA+ETU group than in 
      ETU group (p = 0.025). The thickness (microm) of ASSE (+/- SD) and IE (+/- SD) were 
      measured, respectively: ETU - [27.75 (+/- 0.56) and 18.88 (+/- 0.93)] versus FA+ETU - 
      [28.88 (+/- 0.61) and 21.11 (+/- 0.16)] (p = 0.001). The thickness of IE was 
      significantly enlarged when FA was given (p=0.001). CONCLUSION: Folic acid 
      reduces the number and enlarged the IE of ARMs ETU-induced.
FAU - Faria, Danilo Jose Fiorindo
AU  - Faria DJ
AD  - Universidade Federal de Sao Paulo, BR.
FAU - Simoes, Manuel de Jesus
AU  - Simoes Mde J
AD  - Department of Morphology, UNIFESP, Sao Paulo, SP, BR.
FAU - Martins, Jose Luiz
AU  - Martins JL
AD  - Department of Pediatric Surgery, UNIFESP, Sao Paulo, SP, BR.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 12001-76-2 (Vitamin B Complex)
RN  - 24FOJ4N18S (Ethylenethiourea)
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Anal Canal/abnormalities/embryology
MH  - Animals
MH  - Anorectal Malformations
MH  - Anus, Imperforate/chemically induced/*prevention & control
MH  - Disease Models, Animal
MH  - Ethylenethiourea
MH  - Female
MH  - Fetus/abnormalities
MH  - Folic Acid/*therapeutic use
MH  - Pregnancy
MH  - Random Allocation
MH  - Rats, Wistar
MH  - Rectum/abnormalities/embryology
MH  - Reproducibility of Results
MH  - Vitamin B Complex/*therapeutic use
EDAT- 2015/09/10 06:00
MHDA- 2016/01/21 06:00
CRDT- 2015/09/10 06:00
PHST- 2015/09/10 06:00 [entrez]
PHST- 2015/09/10 06:00 [pubmed]
PHST- 2016/01/21 06:00 [medline]
AID - S0102-86502015000800517 [pii]
AID - 10.1590/S0102-865020150080000001 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2015 Aug;30(8):517-22. doi: 10.1590/S0102-865020150080000001.