PMID- 26354107
OWN - NLM
STAT- MEDLINE
DCOM- 20170126
LR  - 20181113
IS  - 1522-1709 (Electronic)
IS  - 1520-9512 (Linking)
VI  - 20
IP  - 1
DP  - 2016 Mar
TI  - Increased MCP-1 gene expression in monocytes of severe OSA patients and under 
      intermittent hypoxia.
PG  - 425-33
LID - 10.1007/s11325-015-1252-5 [doi]
AB  - PURPOSE: Obstructive sleep apnea (OSA) is known to be a risk factor of coronary 
      artery disease. Monocyte chemoattractant protein-1 (MCP-1), as a critical factor 
      for monocyte infiltration, is known to play a role in the development of 
      atherosclerosis. This study aimed to investigate the effect of intermittent 
      hypoxia, the hallmark of OSA, on the MCP-1 expression of monocytes. METHODS: 
      Peripheral blood was sampled from 61 adults enrolled for suspected OSA. RNA was 
      prepared from the isolated monocytes for the analysis of MCP-1. The effect of in 
      vitro intermittent hypoxia on the regulation and function of MCP-1 was 
      investigated on THP-1 monocytic cells and human monocytes. The mRNA and secreted 
      protein levels were investigated by RT/real-time PCR and enzyme-linked 
      immunosorbent assay, respectively. RESULTS: Monocytic MCP-1 gene expression was 
      found to be increased significantly in severe OSA patients. In vitro intermittent 
      hypoxia was demonstrated to increase the mRNA and protein expression levels of 
      MCP-1 dose- and time-dependently in THP-1 monocytic cells. The MCP-1 mRNA 
      expression in monocytes isolated from OSA patient was induced to a much higher 
      level compared to that from normal control. Pre-treatment with inhibitor for 
      p42/44 MAPK or p38 MAPK suppressed the activation of MCP-1 expression by 
      intermittent hypoxia. CONCLUSIONS: This is the first study to demonstrate the 
      increase of MCP-1 gene expression in monocytes of severe OSA patients. In 
      addition, monocytic MCP-1 gene expression can be induced under intermittent 
      hypoxia.
FAU - Chuang, Li-Pang
AU  - Chuang LP
AD  - Graduate Institute of Clinical Medical Sciences, Chang Gung University, 259 
      Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan.
AD  - Sleep Center, Department of Pulmonary and Critical Care Medicine, Chang Gung 
      Memorial Hospital, Tao-Yuan, Taiwan.
FAU - Chen, Ning-Hung
AU  - Chen NH
AD  - Sleep Center, Department of Pulmonary and Critical Care Medicine, Chang Gung 
      Memorial Hospital, Tao-Yuan, Taiwan.
FAU - Lin, Yuling
AU  - Lin Y
AD  - Graduate Institute of Clinical Medical Sciences, Chang Gung University, 259 
      Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Ko, Wen-Shan
AU  - Ko WS
AD  - Graduate Institute of Clinical Medical Sciences, Chang Gung University, 259 
      Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Pang, Jong-Hwei S
AU  - Pang JH
AD  - Graduate Institute of Clinical Medical Sciences, Chang Gung University, 259 
      Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan. jonghwei@mail.cgu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150909
PL  - Germany
TA  - Sleep Breath
JT  - Sleep & breathing = Schlaf & Atmung
JID - 9804161
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Atherosclerosis/diagnosis/genetics
MH  - Chemokine CCL2/*genetics
MH  - Female
MH  - Gene Expression/genetics
MH  - Humans
MH  - Hypoxia/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Polysomnography
MH  - RNA, Messenger/genetics
MH  - Reference Values
MH  - Risk Factors
MH  - Sleep Apnea, Obstructive/diagnosis/*genetics
OTO - NOTNLM
OT  - Intermittent hypoxia
OT  - Monocyte
OT  - Monocyte chemoattractant protein-1
OT  - Obstructive sleep apnea
EDAT- 2015/09/12 06:00
MHDA- 2017/01/27 06:00
CRDT- 2015/09/11 06:00
PHST- 2015/05/13 00:00 [received]
PHST- 2015/08/25 00:00 [accepted]
PHST- 2015/07/24 00:00 [revised]
PHST- 2015/09/11 06:00 [entrez]
PHST- 2015/09/12 06:00 [pubmed]
PHST- 2017/01/27 06:00 [medline]
AID - 10.1007/s11325-015-1252-5 [pii]
AID - 10.1007/s11325-015-1252-5 [doi]
PST - ppublish
SO  - Sleep Breath. 2016 Mar;20(1):425-33. doi: 10.1007/s11325-015-1252-5. Epub 2015 
      Sep 9.