PMID- 26356872
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150911
LR  - 20201001
IS  - 2225-0719 (Print)
IS  - 2310-8819 (Electronic)
IS  - 2225-0719 (Linking)
VI  - 2
IP  - 4
DP  - 2014 Dec
TI  - Mesenchymal Bone Marrow-derived Stem Cells Transplantation in Patients with HCV 
      Related Liver Cirrhosis.
PG  - 217-21
LID - 10.14218/JCTH.2014.00027 [doi]
AB  - BACKGROUND AND AIMS: To evaluate the effect of intraparenchymal transplantation 
      of mesenchymal bone marrow-derived stem cells (BMSCs) in patients with hepatitis 
      C virus (HCV)-related liver cirrhosis (LC). METHODS: Mononuclear cells were 
      isolated from patient bone marrow and were passaged several times in vitro in 
      order to reach the required volume. Attributes of the BMSCs were evaluated by the 
      presence of the surface markers CD105+, CD90+, and CD73+. Cells from each passage 
      were evaluated for sterility, and they were transplanted intraparenchymally into 
      liver tissue. Clinical and laboratory data were evaluated and morphological 
      studies of liver biopsy were performed prior to and 6 months after 
      transplantation. RESULTS: On clinical evaluation, the general state of these 
      patients was improved at 1 month following transplantation of BMSCs. At 1 and 6 
      months post-transplantation, jaundice was absent in four (67%) patients. After 6 
      months, functional hepatic indices were improved, i.e. decrease of ALT and AST 
      activity and bilirubin level. However, these decreases were not statistically 
      different (P>0.05). Expression of CD34 and alpha-SMA in liver biopsy samples were 
      decreased at 6 months after transplantation, consistent with structural 
      improvements in mitochondria and nuclear compartments. CONCLUSIONS: 
      Intraparenchymal transplantation of autologous BMSCs improved the functional 
      condition of the liver, stimulated reparative processes in hepatocytes, and 
      decreased extracellular matrix protein (EMP) count in hepatic tissues of patients 
      with LC. It was well tolerated and was not associated with any complications both 
      during and after BMSC transplantation.
FAU - Lukashyk, Sviatlana P
AU  - Lukashyk SP
AD  - Department of Infection Diseases, The Belarusian State Medical University, Minsk, 
      Republic of Belarus.
FAU - Tsyrkunov, Vladimir M
AU  - Tsyrkunov VM
AD  - Department of Infection Diseases, The Grodno State Medical University, Grodno, 
      Republic of Belarus.
FAU - Isaykina, Yanina I
AU  - Isaykina YI
AD  - Cell biotechnology and cytotherapy laboratory, The Republican Research and 
      Practical Center of Pediatric Oncology and Hematology, Minsk, Republic of 
      Belarus.
FAU - Romanova, Oksana N
AU  - Romanova ON
AD  - Cell biotechnology and cytotherapy laboratory, The Republican Research and 
      Practical Center of Pediatric Oncology and Hematology, Minsk, Republic of 
      Belarus.
FAU - Shymanskiy, Artur T
AU  - Shymanskiy AT
AD  - Cell biotechnology and cytotherapy laboratory, The Republican Research and 
      Practical Center of Pediatric Oncology and Hematology, Minsk, Republic of 
      Belarus.
FAU - Aleynikova, Olga V
AU  - Aleynikova OV
AD  - Cell biotechnology and cytotherapy laboratory, The Republican Research and 
      Practical Center of Pediatric Oncology and Hematology, Minsk, Republic of 
      Belarus.
FAU - Kravchuk, Rimma I
AU  - Kravchuk RI
AD  - Central Scientific Research Laboratory, The Grodno State Medical University, 
      Grodno, Republic of Belarus.
LA  - eng
PT  - Journal Article
DEP - 20141215
PL  - United States
TA  - J Clin Transl Hepatol
JT  - Journal of clinical and translational hepatology
JID - 101649815
PMC - PMC4521237
OTO - NOTNLM
OT  - Liver cirrhosis
OT  - Mesenchymal bone marrow stem cells
OT  - Transplantation therapy
COIS- Conflict of interest: None
EDAT- 2015/09/12 06:00
MHDA- 2015/09/12 06:01
PMCR- 2014/12/15
CRDT- 2015/09/11 06:00
PHST- 2014/09/02 00:00 [received]
PHST- 2014/11/18 00:00 [revised]
PHST- 2014/11/18 00:00 [accepted]
PHST- 2015/09/11 06:00 [entrez]
PHST- 2015/09/12 06:00 [pubmed]
PHST- 2015/09/12 06:01 [medline]
PHST- 2014/12/15 00:00 [pmc-release]
AID - JCTH440350 [pii]
AID - 10.14218/JCTH.2014.00027 [doi]
PST - ppublish
SO  - J Clin Transl Hepatol. 2014 Dec;2(4):217-21. doi: 10.14218/JCTH.2014.00027. Epub 
      2014 Dec 15.