PMID- 26357961
OWN - NLM
STAT- MEDLINE
DCOM- 20160608
LR  - 20181202
IS  - 1361-6528 (Electronic)
IS  - 0957-4484 (Linking)
VI  - 26
IP  - 39
DP  - 2015 Oct 2
TI  - One-pot synthesis of crosslinked amphiphilic polycarbonates as stable but 
      reduction-sensitive carriers for doxorubicin delivery.
PG  - 395602
LID - 10.1088/0957-4484/26/39/395602 [doi]
AB  - In this paper, we first synthesized a novel disulfide-coupled bis-(cyclic 
      carbonate) (TDCSS) monomer. After ring-opening co-polymerization (ROP) of TDCSS 
      and trimethylene carbonate (TMC) initiated by mono-methoxyl poly(ethylene 
      glycol), the crosslinked reduction-sensitive copolymer PEG-P(TMC-co-TDCSS) was 
      obtained via a facile one-step procedure for efficient delivery of doxorubicin 
      (DOX) into cancer cells. To serve as controls, PEG-P(TMC-co-TDCCC), which has an 
      analogous structure without disulfide bond, and a linear polymer PEG-PTMC were 
      also prepared. The copolymers could self-assemble to form nano-sized micelles in 
      an aqueous solution. As compared to PEG-PTMC, crosslinked PEG-P(TMC-co-TDCSS) and 
      PEG-P(TMC-co-TDCCC) showed lower CMC values and thus induced a much better 
      micelle-forming ability. In vitro release studies revealed that the drug release 
      behavior of DOX-loaded PEG-P(TMC-co-TDCSS) micelles, which could be accelerated 
      in the presence of 10 mM dithiothreitol (DTT), showed a similar trend in the 
      absence of DTT compared to DOX-loaded PEG-P(TMC-co-TDCCC) micelles. Furthermore, 
      confocal laser scanning microscopy (CLSM) indicated that DOX-loaded 
      PEG-P(TMC-co-TDCSS) micelles were efficiently internalized into HeLa cells, 
      releasing DOX into the cytoplasm after which the drug finally entered the nuclei, 
      while MTT assays also demonstrated potent cytotoxic activity against HeLa cells. 
      DOX was mainly located in the cytoplasm for reduction-insensitive 
      PEG-P(TMC-co-TDCCC) and PEG-PTMC controls.
FAU - Yi, Xiaoqing
AU  - Yi X
AD  - Key Laboratory of Biomedical Polymers of Ministry of Education, College of 
      Chemistry and Molecular Science, Wuhan University, Wuhan 430072, People's 
      Republic of China.
FAU - Zhang, Quan
AU  - Zhang Q
FAU - Dong, Hui
AU  - Dong H
FAU - Zhao, Dan
AU  - Zhao D
FAU - Xu, Jia-qi
AU  - Xu JQ
FAU - Zhuo, Renxi
AU  - Zhuo R
FAU - Li, Feng
AU  - Li F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150911
PL  - England
TA  - Nanotechnology
JT  - Nanotechnology
JID - 101241272
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Micelles)
RN  - 0 (Surface-Active Agents)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Antibiotics, Antineoplastic/*administration & dosage/pharmacokinetics
MH  - Doxorubicin/*administration & dosage/pharmacokinetics
MH  - *Drug Delivery Systems
MH  - HeLa Cells
MH  - Humans
MH  - *Micelles
MH  - Molecular Structure
MH  - Nanoparticles/*chemistry/ultrastructure
MH  - Particle Size
MH  - Polyethylene Glycols/*chemistry
MH  - Surface-Active Agents/*chemical synthesis
EDAT- 2015/09/12 06:00
MHDA- 2016/06/09 06:00
CRDT- 2015/09/12 06:00
PHST- 2015/09/12 06:00 [entrez]
PHST- 2015/09/12 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
AID - 10.1088/0957-4484/26/39/395602 [doi]
PST - ppublish
SO  - Nanotechnology. 2015 Oct 2;26(39):395602. doi: 10.1088/0957-4484/26/39/395602. 
      Epub 2015 Sep 11.