PMID- 26359091
OWN - NLM
STAT- MEDLINE
DCOM- 20160107
LR  - 20150911
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 31
IP  - 9
DP  - 2015 Sep
TI  - [TLR4 promotes the activation of B cells in beta2GP1-immunized mice].
PG  - 1156-61
AB  - OBJECTIVE: To investigate the effect of Toll-like receptor 4 (TLR4) on B cell 
      activation in beta2-glycoprotein I (beta2GP1)-immunized mouse models. METHODS: The 
      healthy TLR4+/+ C3H/HeN mice and TLR4-/- C3H/HeJ mice were randomly divided into 
      two groups: beta2GP1 group and normal saline (NS) group. The mice were immunized 
      with human beta2GP1 or equal amount of NS. The primary immunization was done by 
      subcutaneous multi-point injection. The booster immunization was performed by 
      intraperitoneal injection and the last immunization by intravenous injection. The 
      titer of anti-beta2GP1 antibody in mouse sera was evaluated by indirect ELISA. HE 
      staining was used to observe the changes of mouse germinal centers. The 
      expression of CD40 ligand (CD40L) in the germinal centers was detected by 
      immunohistochemistry. And the levels of co-stimulatory molecules CD80 and CD86 in 
      the spleen were determined by flow cytometry. RESULTS: The high titer of 
      anti-beta2GP1 antibody could be detected in mouse sera following immunization with 
      beta2GP1. And the titer of the antibody in TLR4+/+ C3H/HeN mice was higher than that 
      in TLR4-/- C3H/HeJ mice. Compared with NS group, the CD40L, CD19+CD80+ cells and 
      CD19+CD86+ cells in C3H/HeN and C3H/HeJ mice were significantly up-regulated by 
      the treatment of beta2GPI, and the cell numbers in the C3H/HeJ mice were lower than 
      those in the C3H/HeN mice. The germinal centers in beta2GP1-immunized mice were 
      bigger and more than those in NS group, and the number of the germinal centers in 
      TLR4+/+ C3H/HeN mice was more than that in TLR4-/- C3H/HeJ mice. CONCLUSION: TLR4 
      plays an important role in the process of B cell activation in beta2GP1-immunized 
      mouse models.
FAU - Cheng, Si
AU  - Cheng S
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - Xie, Hongxiang
AU  - Xie H
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - Kong, Xiangmin
AU  - Kong X
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - Xie, Yachao
AU  - Xie Y
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - He, Chao
AU  - He C
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - Yu, Yinjing
AU  - Yu Y
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
FAU - Xia, Longfei
AU  - Xia L
AD  - School of Basic Medicine and Medical Technology, Jiangsu University, Zhenjiang 
      212013, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Humans
MH  - Immunization
MH  - *Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Toll-Like Receptor 4/*physiology
MH  - beta 2-Glycoprotein I/*immunology
EDAT- 2015/09/12 06:00
MHDA- 2016/01/08 06:00
CRDT- 2015/09/12 06:00
PHST- 2015/09/12 06:00 [entrez]
PHST- 2015/09/12 06:00 [pubmed]
PHST- 2016/01/08 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Sep;31(9):1156-61.