PMID- 26361715
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20181202
IS  - 1879-0046 (Electronic)
IS  - 0376-8716 (Linking)
VI  - 155
DP  - 2015 Oct 1
TI  - Effects of acute ethanol exposure on class I HDACs family enzymes in wild-type 
      and BDNF(+/-) mice.
PG  - 68-75
LID - S0376-8716(15)01608-7 [pii]
LID - 10.1016/j.drugalcdep.2015.08.015 [doi]
AB  - BACKGROUND: Alterations of brain-derived neurotrophic factor (BDNF) have been 
      associated with the development of addiction to different drugs of abuse, 
      including ethanol (EtOH). EtOH exposure activates the BDNF-signaling cascade in 
      dorsal striatum, which in turn affects further EtOH intake. Different alcohol 
      exposures have been widely demonstrated to modulate chromatin remodeling, 
      affecting histone acetylation/deacetylation balance. Recently, class I histone 
      deacetylases (HDACs) inhibition has been reported to modulate BDNF mRNA 
      expression and to attenuate morphological and behavioral phenomena related to 
      EtOH exposure. However, the role played by different HDAC isoforms in 
      EtOH-induced plasticity is still unclear. METHODS: We investigated the effects 
      induced by acute EtOH exposure on the protein levels of class I HDAC 1-3 isoforms 
      of wild-type (WT) and BDNF heterozygous mice (BDNF(+/-)), in nuclear and 
      cytoplasmic extracts of specific brain regions associated with EtOH addiction. 
      RESULTS: Nuclear HDAC 1-3 levels were markedly reduced after acute EtOH treatment 
      in the caudate putamen (CPu) of WT mice only. Furthermore, CPu basal levels of 
      nuclear HDAC isoforms were significantly lower in BDNF(+/-) mice compared to WT. 
      With the exception of nuclear HDAC 3, no significant changes were observed after 
      acute EtOH treatment in the prefrontal cortex (PFCx) of BDNF(+/-) and WT mice. In 
      this area, the nuclear HDAC basal levels were significantly different between the 
      two experimental groups. CONCLUSIONS: These results provide details about EtOH 
      effects on class I HDAC isoforms and strongly support a correlation between BDNF 
      and class I HDACs, suggesting a possible influence of BNDF on these enzymes.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Caputi, F F
AU  - Caputi FF
AD  - Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of 
      Bologna, Irnerio 48, 40126 Bologna, Italy.
FAU - Palmisano, M
AU  - Palmisano M
AD  - Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of 
      Bologna, Irnerio 48, 40126 Bologna, Italy.
FAU - D'Addario, C
AU  - D'Addario C
AD  - Faculty of Bioscience and Technology for Food, Agriculture and Environment, 
      University of Teramo, Teramo, Italy; Karolinska Institutet, Department of 
      Clinical Neuroscience, Center for Molecular Medicine, Stockholm, Sweden.
FAU - Candeletti, S
AU  - Candeletti S
AD  - Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of 
      Bologna, Irnerio 48, 40126 Bologna, Italy.
FAU - Romualdi, P
AU  - Romualdi P
AD  - Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of 
      Bologna, Irnerio 48, 40126 Bologna, Italy. Electronic address: 
      patrizia.romualdi@unibo.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150828
PL  - Ireland
TA  - Drug Alcohol Depend
JT  - Drug and alcohol dependence
JID - 7513587
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Isoenzymes)
RN  - 3K9958V90M (Ethanol)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cell Nucleus/drug effects/enzymology
MH  - Cytoplasm/drug effects/enzymology
MH  - Ethanol/*administration & dosage/*pharmacology
MH  - Female
MH  - Heterozygote
MH  - Histone Deacetylases/*metabolism
MH  - Isoenzymes/metabolism
MH  - Male
MH  - Mice
MH  - Prefrontal Cortex/drug effects/enzymology
MH  - Putamen/drug effects/enzymology
MH  - Rotarod Performance Test
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Ethanol
OT  - Histone deacetylases
EDAT- 2015/09/13 06:00
MHDA- 2016/05/10 06:00
CRDT- 2015/09/13 06:00
PHST- 2015/04/01 00:00 [received]
PHST- 2015/07/21 00:00 [revised]
PHST- 2015/08/17 00:00 [accepted]
PHST- 2015/09/13 06:00 [entrez]
PHST- 2015/09/13 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - S0376-8716(15)01608-7 [pii]
AID - 10.1016/j.drugalcdep.2015.08.015 [doi]
PST - ppublish
SO  - Drug Alcohol Depend. 2015 Oct 1;155:68-75. doi: 10.1016/j.drugalcdep.2015.08.015. 
      Epub 2015 Aug 28.