PMID- 26362885
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20211203
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 308
DP  - 2015 Nov 12
TI  - mTOR signaling controls VGLUT2 expression to maintain pain hypersensitivity after 
      tissue injury.
PG  - 169-79
LID - S0306-4522(15)00823-4 [pii]
LID - 10.1016/j.neuroscience.2015.09.013 [doi]
AB  - Mammalian target of rapamycin (mTOR) is a serine-threonine protein kinase that 
      controls protein synthesis in the nervous system. Here, we characterized the role 
      of protein synthesis regulation due to mTOR signaling in rat dorsal root ganglion 
      (DRG) following plantar incision. The number of phosphorylated mTOR 
      (p-mTOR)-positive neurons was increased 2-4days after the incision. Rapamycin 
      inhibited p-mTOR expression in the DRG and thermal hypersensitivity 3days but not 
      1day after the incision. Vesicular glutamate transporter 2 (VGLUT2) expression 
      was increased after the plantar incision, which was inhibited by rapamycin. These 
      results demonstrated that tissue injury induces phosphorylation of mTOR and 
      increased protein level of VGLUT2 in the DRG neurons. mTOR phosphorylation 
      involves in maintenance of injury-induced thermal hypersensitivity.
CI  - Copyright (c) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Izumi, Y
AU  - Izumi Y
AD  - Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
FAU - Sasaki, M
AU  - Sasaki M
AD  - Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
FAU - Hashimoto, S
AU  - Hashimoto S
AD  - Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
FAU - Sawa, T
AU  - Sawa T
AD  - Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
FAU - Amaya, F
AU  - Amaya F
AD  - Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan. 
      Electronic address: ama@koto.kpu-m.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150908
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Analgesics)
RN  - 0 (Slc17a6 protein, rat)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpv1 protein, rat)
RN  - 0 (Vesicular Glutamate Transport Protein 2)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Analgesics/pharmacology
MH  - Animals
MH  - Disease Models, Animal
MH  - Foot Injuries
MH  - Ganglia, Spinal/drug effects/*metabolism/pathology
MH  - Hindlimb/injuries
MH  - Hot Temperature
MH  - Hyperalgesia/drug therapy/etiology/*metabolism/pathology
MH  - Male
MH  - Neurons/drug effects/*metabolism/pathology
MH  - Pain/drug therapy/*metabolism/pathology
MH  - Pain Threshold/drug effects/physiology
MH  - Phosphorylation
MH  - Posterior Horn Cells/drug effects/metabolism/pathology
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
MH  - TRPV Cation Channels/metabolism
MH  - Vesicular Glutamate Transport Protein 2/*metabolism
OTO - NOTNLM
OT  - VGLUT2
OT  - hyperalgesia
OT  - incision
OT  - mTOR
EDAT- 2015/09/13 06:00
MHDA- 2016/07/20 06:00
CRDT- 2015/09/13 06:00
PHST- 2015/05/20 00:00 [received]
PHST- 2015/08/16 00:00 [revised]
PHST- 2015/09/03 00:00 [accepted]
PHST- 2015/09/13 06:00 [entrez]
PHST- 2015/09/13 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
AID - S0306-4522(15)00823-4 [pii]
AID - 10.1016/j.neuroscience.2015.09.013 [doi]
PST - ppublish
SO  - Neuroscience. 2015 Nov 12;308:169-79. doi: 10.1016/j.neuroscience.2015.09.013. 
      Epub 2015 Sep 8.