PMID- 26364917
OWN - NLM
STAT- MEDLINE
DCOM- 20170710
LR  - 20181113
IS  - 2042-0226 (Electronic)
IS  - 1672-7681 (Print)
IS  - 1672-7681 (Linking)
VI  - 14
IP  - 3
DP  - 2017 Mar
TI  - Screening and identification of mimotopes of the major shrimp allergen 
      tropomyosin using one-bead-one-compound peptide libraries.
PG  - 308-318
LID - 10.1038/cmi.2015.83 [doi]
AB  - The one-bead-one-compound (OBOC) combinatorial peptide library is a powerful tool 
      to identify ligand and receptor interactions. Here, we applied the OBOC library 
      technology to identify mimotopes specific to the immunoglobulin E (IgE) epitopes 
      of the major shellfish allergen tropomyosin. OBOC peptide libraries with 8-12 
      amino acid residues were screened with serum samples from patients with shellfish 
      allergy for IgE mimotopes of tropomyosin. Twenty-five mimotopes were identified 
      from the screening and their binding reactivity to tropomyosin-specific IgE was 
      confirmed by peptide ELISA. These mimotopes could be divided into seven clusters 
      based on sequence homology, and epitope mapping by EpiSearch of the clustered 
      mimotopes was performed to characterize and confirm the validity of mimotopes. 
      Five out of six of the predicted epitopes were found to overlap with previously 
      identified epitopes of tropomyosin. To further confirm the mimicry potential of 
      mimotopes, BALB/c mice were immunized with mimotopes conjugated to keyhole limpet 
      hemocyanin and assayed for their capacity to induce tropomyosin-specific 
      antibodies. BALB/c mice that received mimotope immunization were found to have an 
      elevated level of tropomyosin-specific immunoglobulin G, but not mice that 
      received an irrelevant mimotope. This study pioneers the successful application 
      of the OBOC libraries using whole sera to screen and identify multiple shrimp 
      allergen mimotopes and validates their mimicry potential using in vitro, in vivo, 
      and in silico methods.Cellular & Molecular Immunology advance online publication, 
      14 september 2015; doi:10.1038/cmi.2015.83.
FAU - Leung, Nicki Yh
AU  - Leung NY
AD  - School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong 
      Kong SAR, China.
FAU - Wai, Christine Yy
AU  - Wai CY
AD  - School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong 
      Kong SAR, China.
FAU - Ho, Marco Hk
AU  - Ho MH
AD  - Department of Pediatrics and Adolescent Medicine, Queen Mary Hospital, The 
      University of Hong Kong, Pokfulam, Hong Kong SAR, China.
FAU - Liu, Ruiwu
AU  - Liu R
AD  - Department of Biochemistry and Molecular Medicine, University of California, 
      Davis, CA 95616, USA.
FAU - Lam, Kit S
AU  - Lam KS
AD  - Department of Biochemistry and Molecular Medicine, University of California, 
      Davis, CA 95616, USA.
FAU - Wang, Jin Jun
AU  - Wang JJ
AD  - Division of Rheumatology/Allergy, School of Medicine, University of California, 
      Davis, CA 95616, USA.
FAU - Shu, Shang An
AU  - Shu SA
AD  - Division of Rheumatology/Allergy, School of Medicine, University of California, 
      Davis, CA 95616, USA.
FAU - Chu, Ka Hou
AU  - Chu KH
AD  - School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong 
      Kong SAR, China.
FAU - Leung, Patrick Sc
AU  - Leung PS
AD  - Division of Rheumatology/Allergy, School of Medicine, University of California, 
      Davis, CA 95616, USA.
LA  - eng
GR  - P30 CA093373/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20150914
PL  - China
TA  - Cell Mol Immunol
JT  - Cellular & molecular immunology
JID - 101242872
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Cell Mol Immunol. 2019 May;16(5):522. PMID: 30374146
MH  - Allergens/*immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Combinatorial Chemistry Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitope Mapping
MH  - Epitopes/chemistry/*immunology
MH  - Humans
MH  - Immunization
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - *Mass Screening
MH  - Mice, Inbred BALB C
MH  - *Microspheres
MH  - Models, Molecular
MH  - Penaeidae/*immunology
MH  - *Peptide Library
MH  - Peptides/immunology
MH  - Structural Homology, Protein
MH  - Tropomyosin/*immunology
PMC - PMC5360880
EDAT- 2015/09/15 06:00
MHDA- 2017/07/14 06:00
PMCR- 2018/03/01
CRDT- 2015/09/15 06:00
PHST- 2015/05/22 00:00 [received]
PHST- 2015/08/06 00:00 [revised]
PHST- 2015/08/06 00:00 [accepted]
PHST- 2015/09/15 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2015/09/15 06:00 [entrez]
PHST- 2018/03/01 00:00 [pmc-release]
AID - cmi201583 [pii]
AID - 10.1038/cmi.2015.83 [doi]
PST - ppublish
SO  - Cell Mol Immunol. 2017 Mar;14(3):308-318. doi: 10.1038/cmi.2015.83. Epub 2015 Sep 
      14.