PMID- 26365167
OWN - NLM
STAT- MEDLINE
DCOM- 20160727
LR  - 20151020
IS  - 1618-0607 (Electronic)
IS  - 1438-4221 (Linking)
VI  - 305
IP  - 7
DP  - 2015 Oct
TI  - Fluorescence in situ hybridization for the identification of Treponema pallidum 
      in tissue sections.
PG  - 709-18
LID - S1438-4221(15)00085-5 [pii]
LID - 10.1016/j.ijmm.2015.08.022 [doi]
AB  - Syphilis is often called the great imitator because of its frequent atypical 
      clinical manifestations that make the disease difficult to recognize. Because 
      Treponema pallidum subsp. pallidum, the infectious agent of syphilis, is yet 
      uncultivated in vitro, diagnosis is usually made using serology; however, in 
      cases where serology is inconclusive or in patients with immunosuppression where 
      these tests may be difficult to interpret, the availability of a molecular tool 
      for direct diagnosis may be of pivotal importance. Here we present a fluorescence 
      in situ hybridization (FISH) assay that simultaneously identifies and analyzes 
      spatial distribution of T. pallidum in histological tissue sections. For this 
      assay the species-specific FISH probe TPALL targeting the 16S rRNA of T. pallidum 
      was designed in silico and evaluated using T. pallidum infected rabbit testicular 
      tissue and a panel of non-syphilis spirochetes as positive and negative controls, 
      respectively, before application to samples from four syphilis-patients. In a HIV 
      positive patient, FISH showed the presence of T. pallidum in inguinal lymph node 
      tissue. In a patient not suspected to suffer from syphilis but underwent surgery 
      for phimosis, numerous T. pallidum cells were found in preputial tissue. In two 
      cases with oral involvement, FISH was able to differentiate T. pallidum from oral 
      treponemes and showed infection of the oral mucosa and tonsils, respectively. The 
      TPALL FISH probe is now readily available for in situ identification of T. 
      pallidum in selected clinical samples as well as T. pallidum research 
      applications and animal models.
CI  - Copyright (c) 2015 Elsevier GmbH. All rights reserved.
FAU - Petrich, Annett
AU  - Petrich A
AD  - Institute for Microbiology and Hygiene, Charite-Universitatsmedizin Berlin, 
      Berlin, Germany.
FAU - Rojas, Pablo
AU  - Rojas P
AD  - Institute for Microbiology and Hygiene, Charite-Universitatsmedizin Berlin, 
      Berlin, Germany.
FAU - Schulze, Julia
AU  - Schulze J
AD  - Biofilmzentrum, Deutsches Herzzentrum Berlin, Consultant Laboratory for 
      Tropheryma whipplei, Berlin, Germany.
FAU - Loddenkemper, Christoph
AU  - Loddenkemper C
AD  - Institute for Pathology, Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Giacani, Lorenzo
AU  - Giacani L
AD  - Department of Medicine, Division of Allergy and Infectious Diseases, and 
      Department of Global Health, University of Washington, Seattle, WA 98104, USA.
FAU - Schneider, Thomas
AU  - Schneider T
AD  - Department of Gastroenterology, Infectiology and Rheumatology, 
      Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Hertel, Moritz
AU  - Hertel M
AD  - Department of Oral Medicine, Dental Radiology and Oral Surgery, 
      Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Institute for Microbiology and Hygiene, Charite-Universitatsmedizin Berlin, 
      Berlin, Germany; Biofilmzentrum, Deutsches Herzzentrum Berlin, Consultant 
      Laboratory for Tropheryma whipplei, Berlin, Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmzentrum, Deutsches Herzzentrum Berlin, Consultant Laboratory for 
      Tropheryma whipplei, Berlin, Germany. Electronic address: moter@dhzb.de.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20150828
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - DNA, Bacterial/*analysis/genetics
MH  - DNA, Ribosomal/genetics
MH  - Disease Models, Animal
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymph Nodes/microbiology/pathology
MH  - Male
MH  - Middle Aged
MH  - Mouth Mucosa/microbiology/pathology
MH  - Oligonucleotide Probes/genetics
MH  - Palatine Tonsil/microbiology/pathology
MH  - Pathology, Molecular/*methods
MH  - Penis/microbiology/pathology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rabbits
MH  - Syphilis/*diagnosis/*pathology
MH  - Testis/microbiology/pathology
MH  - Treponema pallidum/*genetics
OTO - NOTNLM
OT  - Fluorescence in situ hybridization (FISH)
OT  - Syphilis
OT  - Treponema pallidum subsp. pallidum
EDAT- 2015/09/15 06:00
MHDA- 2016/07/28 06:00
CRDT- 2015/09/15 06:00
PHST- 2015/09/15 06:00 [entrez]
PHST- 2015/09/15 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - S1438-4221(15)00085-5 [pii]
AID - 10.1016/j.ijmm.2015.08.022 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2015 Oct;305(7):709-18. doi: 10.1016/j.ijmm.2015.08.022. 
      Epub 2015 Aug 28.