PMID- 26365343
OWN - NLM
STAT- MEDLINE
DCOM- 20160512
LR  - 20151012
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 140
IP  - 21
DP  - 2015 Nov 7
TI  - An 8 minute colorimetric paper-based reverse phase vertical flow serum microarray 
      for screening of hyper IgE syndrome.
PG  - 7327-34
LID - 10.1039/c5an01013f [doi]
AB  - Reverse phase microarrays are useful tools for affinity-based detection in 
      hundreds of samples simultaneously. However, current methods typically require 
      long assay times and fluorescent detection. Here we describe a paper-based 
      Vertical Flow Microarray (VFM) assay as a rapid 8-minute colorimetric alternative 
      for reverse phase microarray analysis. The VFM platform was optimized for 
      detection of IgE with a detection limit of 1.9 mug mL(-1) in whole serum. 
      Optimized conditions were then used to screen 113 serum samples simultaneously 
      for hyper IgE syndrome (hIgE), a rare primary immunodeficiency characterized by 
      elevated levels of IgE. The same set of samples were then analysed with a 
      conventional planar microarray with fluorescent detection for head-to-head 
      testing. Both assays found elevated levels in three out of four hIgE patient 
      samples, whereas no control samples displayed elevated levels in either method. 
      The comparison experiments showed a good correlation between the two assays, as 
      determined from a linear correlation study (Pearson's r = 0.76). Further, the 
      assay-time reduction and reproducibility (intra assay CV = 12.4 +/- 4.11%) 
      demonstrate the applicability of the VFM platform for high throughput reverse 
      phase screening.
FAU - Reutersward, Philippa
AU  - Reutersward P
AD  - Division of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH 
      Royal Institute of Technology, Sweden. helene.andersson.svahn@scilifelab.se.
FAU - Gantelius, Jesper
AU  - Gantelius J
FAU - Andersson Svahn, Helene
AU  - Andersson Svahn H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9004-70-0 (Collodion)
SB  - IM
MH  - Area Under Curve
MH  - Collodion/chemistry
MH  - Colorimetry/*instrumentation/*methods
MH  - Equipment Design
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Job Syndrome/*blood/*diagnosis
MH  - Limit of Detection
MH  - Microarray Analysis
MH  - Paper
MH  - Protein Array Analysis/methods
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2015/09/15 06:00
MHDA- 2016/05/14 06:00
CRDT- 2015/09/15 06:00
PHST- 2015/09/15 06:00 [entrez]
PHST- 2015/09/15 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
AID - 10.1039/c5an01013f [doi]
PST - ppublish
SO  - Analyst. 2015 Nov 7;140(21):7327-34. doi: 10.1039/c5an01013f.