PMID- 26365345
OWN - NLM
STAT- MEDLINE
DCOM- 20160111
LR  - 20151005
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 466
IP  - 3
DP  - 2015 Oct 23
TI  - Amphiregulin may be a new biomarker of classically activated macrophages.
PG  - 393-9
LID - S0006-291X(15)30554-4 [pii]
LID - 10.1016/j.bbrc.2015.09.037 [doi]
AB  - Amphiregulin (Areg) participates in tissue repair and inflammation regulation. As 
      important effector cells in inflammation, macrophages can be polarized to 
      classically (M1) or alternatively (M2) activated phenotype with diverse functions 
      in immunity. However, the relationship between Areg expression and macrophage 
      activation is poorly understood. Here we report that Areg was significantly 
      expressed in M1 but not in M2 macrophages. This was confirmed by analyses of 
      RT-PCR and ELISA in peritoneal macrophages, and by evaluating protein expression 
      in alveolar macrophages and RAW264.7 cells. Selective inhibitors of TLR4 
      (CLI-095) and MAP kinase, including Erk1/2 (PD98059), JNK (SP600125) and p38 
      (SB203580), significantly reduced Areg expression in M1 macrophages, suggesting 
      that M1 macrophages produce Areg mainly through the TLR4-MAPK pathway, which is 
      involved in the mechanism of M1 activation. When compared with productions of 
      classical biomarkers of M1 macrophages, Areg expression was highly consistent in 
      time series. Taken together, Areg may be an effective new biomarker of M1 
      macrophages.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Meng, Chen
AU  - Meng C
AD  - Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China. Electronic address: 
      27739865@qq.com.
FAU - Liu, Guilin
AU  - Liu G
AD  - Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China. Electronic address: 
      524397325@qq.com.
FAU - Mu, Honglan
AU  - Mu H
AD  - Department of Internal Medicine, Tongji Medical College Hospital, Huazhong 
      University of Science and Technology, Wuhan, China. Electronic address: 
      126071497@qq.com.
FAU - Zhou, Miaomiao
AU  - Zhou M
AD  - Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan, China. 
      Electronic address: zhoumiaomiao09@aliyun.com.
FAU - Zhang, Shihai
AU  - Zhang S
AD  - Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China. Electronic address: 
      zhangshihai@vip.163.com.
FAU - Xu, Younian
AU  - Xu Y
AD  - Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China. Electronic address: 
      xyn0103@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150910
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amphiregulin)
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Amphiregulin/*metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Epidermal Growth Factor/metabolism
MH  - Gene Expression Regulation
MH  - Inflammation/metabolism
MH  - Macrophage Activation
MH  - Macrophages/*metabolism
MH  - Macrophages, Alveolar/*metabolism
MH  - Macrophages, Peritoneal/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Signal Transduction
MH  - Th2 Cells/cytology
MH  - Time Factors
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - Amphiregulin
OT  - Epidermal growth factor
OT  - Macrophage activation
OT  - Macrophages
OT  - Receptor
EDAT- 2015/09/15 06:00
MHDA- 2016/01/12 06:00
CRDT- 2015/09/15 06:00
PHST- 2015/09/01 00:00 [received]
PHST- 2015/09/07 00:00 [accepted]
PHST- 2015/09/15 06:00 [entrez]
PHST- 2015/09/15 06:00 [pubmed]
PHST- 2016/01/12 06:00 [medline]
AID - S0006-291X(15)30554-4 [pii]
AID - 10.1016/j.bbrc.2015.09.037 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Oct 23;466(3):393-9. doi: 
      10.1016/j.bbrc.2015.09.037. Epub 2015 Sep 10.