PMID- 26365514
OWN - NLM
STAT- MEDLINE
DCOM- 20160802
LR  - 20181113
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 5
IP  - 4
DP  - 2015 Oct 13
TI  - CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver 
      Progenitor Cells Derived from Human iPS Cells.
PG  - 508-15
LID - S2213-6711(15)00242-8 [pii]
LID - 10.1016/j.stemcr.2015.08.008 [doi]
AB  - To develop a culture system for large-scale production of mature hepatocytes, 
      liver progenitor cells (LPCs) with a high proliferation potential would be 
      advantageous. We have found that carboxypeptidase M (CPM) is highly expressed in 
      embryonic LPCs, hepatoblasts, while its expression is decreased along with 
      hepatic maturation. Consistently, CPM expression was transiently induced during 
      hepatic specification from human-induced pluripotent stem cells (hiPSCs). CPM(+) 
      cells isolated from differentiated hiPSCs at the immature hepatocyte stage 
      proliferated extensively in vitro and expressed a set of genes that were typical 
      of hepatoblasts. Moreover, the CPM(+) cells exhibited a mature hepatocyte 
      phenotype after induction of hepatic maturation and also underwent cholangiocytic 
      differentiation in a three-dimensional culture system. These results indicated 
      that hiPSC-derived CPM(+) cells share the characteristics of LPCs, with the 
      potential to proliferate and differentiate bi-directionally. Thus, CPM is a 
      useful marker for isolating hiPSC-derived LPCs, which allows development of a 
      large-scale culture system for producing hepatocytes and cholangiocytes.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Kido, Taketomo
AU  - Kido T
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. Electronic 
      address: kido@iam.u-tokyo.ac.jp.
FAU - Koui, Yuta
AU  - Koui Y
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Suzuki, Kaori
AU  - Suzuki K
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan; Department 
      of Host Defense and Biochemical Research, Juntendo University, School of 
      Medicine, Tokyo 113-8421, Japan.
FAU - Kobayashi, Ayaka
AU  - Kobayashi A
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Miura, Yasushi
AU  - Miura Y
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Chern, Edward Y
AU  - Chern EY
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan; Department 
      of Biochemical Science and Technology, National Taiwan University, Taipei 106, 
      Taiwan.
FAU - Tanaka, Minoru
AU  - Tanaka M
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Miyajima, Atsushi
AU  - Miyajima A
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. Electronic 
      address: miyajima@iam.u-tokyo.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150910
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (GPI-Linked Proteins)
RN  - EC 3.4.17.12 (carboxypeptidase M)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Embryonic Stem Cells/cytology
MH  - GPI-Linked Proteins/analysis
MH  - Hepatocytes/*cytology
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology
MH  - Metalloendopeptidases/*analysis
MH  - Mice
PMC - PMC4624956
EDAT- 2015/09/15 06:00
MHDA- 2016/08/03 06:00
PMCR- 2015/09/10
CRDT- 2015/09/15 06:00
PHST- 2015/04/08 00:00 [received]
PHST- 2015/08/07 00:00 [revised]
PHST- 2015/08/08 00:00 [accepted]
PHST- 2015/09/15 06:00 [entrez]
PHST- 2015/09/15 06:00 [pubmed]
PHST- 2016/08/03 06:00 [medline]
PHST- 2015/09/10 00:00 [pmc-release]
AID - S2213-6711(15)00242-8 [pii]
AID - 10.1016/j.stemcr.2015.08.008 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2015 Oct 13;5(4):508-15. doi: 10.1016/j.stemcr.2015.08.008. 
      Epub 2015 Sep 10.