PMID- 26365696
OWN - NLM
STAT- MEDLINE
DCOM- 20161024
LR  - 20191210
IS  - 1873-2933 (Electronic)
IS  - 0009-9120 (Print)
IS  - 0009-9120 (Linking)
VI  - 49
IP  - 1-2
DP  - 2016 Jan
TI  - Development and validation of an immunosensor for monocyte chemotactic protein 1 
      using a silicon photonic microring resonator biosensing platform.
PG  - 121-6
LID - S0009-9120(15)00431-2 [pii]
LID - 10.1016/j.clinbiochem.2015.09.001 [doi]
AB  - OBJECTIVES: We report the development of an optical immunosensor for the 
      detection of monocyte chemotactic protein 1 (MCP-1) in serum samples. MCP-1 is a 
      cytokine that is an emerging biomarker for several diseases/disorders, including 
      ischemic cardiomyopathy, fibromyalgia, and some cancers. DESIGN AND METHODS: The 
      detection of MCP-1 was achieved by performing a sandwich immunoassay on a silicon 
      photonic microring resonator sensor platform. The resonance wavelengths supported 
      by microring sensors are responsive to local changes in the environment 
      accompanying biomarker binding. This technology offers a modularly multiplexable 
      approach to detecting analyte localization in an antibody-antigen complex at the 
      sensor surface. RESULTS: The immunosensor allowed the rapid detection of MCP-1 in 
      buffer and spiked human serum samples. An almost 2 order of magnitude linear 
      range was observed, between 84.3 and 1582.1pg/mL and the limits of blank and 
      detection were determined to be 0.3 and 0.5pg/mL, respectively. The platform's 
      ability to analyze MCP-1 concentrations across a clinically-relevant 
      concentration range was demonstrated. CONCLUSIONS: A silicon photonic 
      immunosensor technology was applied to the detection of clinically-relevant 
      concentrations of MCP-1. The performance of the sensor was validated through a 
      broad dynamic range and across a number of suggested clinical cut-off values. 
      Importantly, the intrinsic scalability and rapidity of the technology makes it 
      readily amenable to the simultaneous detection of multiplexed biomarker panels, 
      which is particularly needed for the clinical realization of inflammatory 
      diagnostics.
CI  - Copyright (c) 2015 The Canadian Society of Clinical Chemists. Published by Elsevier 
      Inc. All rights reserved.
FAU - Valera, Enrique
AU  - Valera E
AD  - Department of Chemistry, University of Illinois at Urbana - Champaign, 600 South 
      Matthews Avenue, Urbana, IL 61801, United States.
FAU - Shia, Winnie W
AU  - Shia WW
AD  - Department of Chemistry, University of Illinois at Urbana - Champaign, 600 South 
      Matthews Avenue, Urbana, IL 61801, United States.
FAU - Bailey, Ryan C
AU  - Bailey RC
AD  - Department of Chemistry, University of Illinois at Urbana - Champaign, 600 South 
      Matthews Avenue, Urbana, IL 61801, United States. Electronic address: 
      baileyrc@illinois.edu.
LA  - eng
GR  - R33 CA177462/CA/NCI NIH HHS/United States
GR  - R33-CA177462-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20150910
PL  - United States
TA  - Clin Biochem
JT  - Clinical biochemistry
JID - 0133660
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Biomarkers/*blood
MH  - *Biosensing Techniques
MH  - Chemokine CCL2/*blood
MH  - Fibromyalgia/blood/diagnosis
MH  - Humans
MH  - Limit of Detection
MH  - Myocardial Ischemia/blood/diagnosis
MH  - Neoplasms/blood/diagnosis
MH  - Photons
PMC - PMC4715927
MID - NIHMS726161
OTO - NOTNLM
OT  - Cytokine
OT  - Diagnostic
OT  - Immunoassay
OT  - Optical biosensor
OT  - Silicon photonics
EDAT- 2015/09/15 06:00
MHDA- 2016/10/25 06:00
PMCR- 2017/01/01
CRDT- 2015/09/15 06:00
PHST- 2015/06/06 00:00 [received]
PHST- 2015/09/03 00:00 [revised]
PHST- 2015/09/06 00:00 [accepted]
PHST- 2015/09/15 06:00 [entrez]
PHST- 2015/09/15 06:00 [pubmed]
PHST- 2016/10/25 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - S0009-9120(15)00431-2 [pii]
AID - 10.1016/j.clinbiochem.2015.09.001 [doi]
PST - ppublish
SO  - Clin Biochem. 2016 Jan;49(1-2):121-6. doi: 10.1016/j.clinbiochem.2015.09.001. 
      Epub 2015 Sep 10.