PMID- 26368612
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150916
LR  - 20150915
IS  - 0895-8378 (Print)
IS  - 0895-8378 (Linking)
VI  - 12 Suppl 3
DP  - 2000 Jan
TI  - Occupational and Environmental Factors Enhancing the Genotoxicity of Asbestos.
PG  - 157-65
LID - 10.1080/08958378.2000.11463209 [doi]
AB  - Epidemiological and experimental studies have suggested the enhancement of 
      asbestos-induced disease processes by simultaneous exposure to kerosene, its 
      soot, and cigarette smoke in asbestos-exposed animals as well as in humans. To 
      determine the influence of these factors on the genotoxic potential of asbestos, 
      a micronucleus test was performed in Syrian hamster embryo fibroblasts (SHE) and 
      human lymphocytes. To observe the specific chromosomal damages, multicolor 
      fluorescence in situ hybridization (FISH) was done in the lymphocytes from 
      smokers and nonsmokers exposed in vitro to asbestos. Significantly higher numbers 
      of micronuclei were observed in SHE cells after combined treatment with 
      chrysotile and kerosene soot (111 micronuclei/1000 cells) in comparison to 
      chrysotile and kerosene soot separately. Kinetochore staining revealed mainly 
      clastogenic effects in all the cases. In human lymphocytes exposed in cultures to 
      chrysotile and crocidolite the numbers of micronuclei were found higher in 
      smokers than nonsmokers. Multicolor FISH assay suggested that asbestos fibers 
      inflict high damage within 1q12 and in the region between 1cen and 1q12 of 
      chromosome 1. In the exposed population of an asbestos cement factory, the 
      highest genetic damage was found in the blood lymphocytes of exposed smokers. The 
      study suggests that smokers occupationally exposed to asbestos and domestically 
      to kerosene soot are at higher risk for the early development of asbestos-induced 
      diseases.
FAU - Rahman, Q
AU  - Rahman Q
AD  - a Industrial Toxicology Research Center, Division of Fiber Toxicology , Lucknow , 
      India.
FAU - Dopp, E
AU  - Dopp E
AD  - b Institute of Animal Physiology, Department of Biology, University of Rostock , 
      Rostock , Germany.
FAU - Lohani, M
AU  - Lohani M
AD  - a Industrial Toxicology Research Center, Division of Fiber Toxicology , Lucknow , 
      India.
FAU - Schiffmann, D
AU  - Schiffmann D
AD  - b Institute of Animal Physiology, Department of Biology, University of Rostock , 
      Rostock , Germany.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
EDAT- 2000/01/01 00:00
MHDA- 2000/01/01 00:01
CRDT- 2015/09/15 06:00
PHST- 2015/09/15 06:00 [entrez]
PHST- 2000/01/01 00:00 [pubmed]
PHST- 2000/01/01 00:01 [medline]
AID - 10.1080/08958378.2000.11463209 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2000 Jan;12 Suppl 3:157-65. doi: 10.1080/08958378.2000.11463209.