PMID- 26368637
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150916
LR  - 20150915
IS  - 0895-8378 (Print)
IS  - 0895-8378 (Linking)
VI  - 12 Suppl 3
DP  - 2000 Jan
TI  - IgE Adjuvant Activity of Particles-What Physical Characteristics are Important?
PG  - 365-72
LID - 10.1080/08958378.2000.11463247 [doi]
AB  - Diesel exhaust particles (DEP) are reported to increase the allergic immune 
      response to ovalbumin (OVA) and pollen. There are studies reporting that both the 
      adsorbed chemical substances and the carbon core of DEP may contribute to the 
      immunoglobulin E (IgE) adjuvant effect. The aim of this study was to investigate 
      which physical properties of particles per se, that is, the particles by 
      themselves, might be important for their IgE adjuvant effect, namely, dose 
      weight, size, number, and surface area. Since DEP have a tendency to form 
      aggregates of varying shapes and sizes, evaluation of the relative importance of 
      these characteristics is difficult using DEP. We therefore decided to use 
      well-characterized, spherical polystyrene particles (PSP). We performed four 
      different types of experiments, in which the total dose weight (12.25 mg), size 
      (0.1 mum in diameter), total number (8 x 10(10)), or total surface area (1300 
      cm(2)) of PSP, respectively, was kept constant. NIH/Ola mice were given 2 
      intraperitoneal injections with PSP plus OVA or OVA alone, over a 16-day period. 
      The mice were exsanguinated at the end of each experiment, and the serum 
      concentration of IgE anti-OVA was measured. The serum levels of IgE anti-OVA 
      increased with both an increasing number and surface area of PSP. We found no 
      clear association between PSP size and the levels of IgE anti-OVA, but because of 
      the relatively small size range of PSP used, no definitive conclusions can be 
      made on whether size is an important factor for the IgE adjuvant effect of PSP. 
      There seemed to be no covariation between the dose weight and the levels of IgE 
      anti-OVA. Our findings indicate that the total number and total surface area of 
      PSP, rather than the dose weight, are important parameters for the IgE adjuvant 
      activity from PSP, and possibly also for particles in general.
FAU - Granum, B
AU  - Granum B
AD  - a Department of Environmental Medicine , National Institute of Public Health , 
      Oslo , Norway.
FAU - Gaarder, P I
AU  - Gaarder PI
AD  - b Department of Immunology and Transfusion Medicine , Ulleval University Hospital 
      , Oslo , Norway.
FAU - Lovik, M
AU  - Lovik M
AD  - a Department of Environmental Medicine , National Institute of Public Health , 
      Oslo , Norway.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
EDAT- 2000/01/01 00:00
MHDA- 2000/01/01 00:01
CRDT- 2015/09/15 06:00
PHST- 2015/09/15 06:00 [entrez]
PHST- 2000/01/01 00:00 [pubmed]
PHST- 2000/01/01 00:01 [medline]
AID - 10.1080/08958378.2000.11463247 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2000 Jan;12 Suppl 3:365-72. doi: 10.1080/08958378.2000.11463247.