PMID- 26369908
OWN - NLM
STAT- MEDLINE
DCOM- 20160909
LR  - 20181113
IS  - 1460-2423 (Electronic)
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 26
IP  - 1
DP  - 2016 Jan
TI  - Identification and biological consequences of the O-GlcNAc modification of the 
      human innate immune receptor, Nod2.
PG  - 13-8
LID - 10.1093/glycob/cwv076 [doi]
AB  - Nucleotide-binding oligomerization domain 2 (Nod2) is an intracellular receptor 
      that can sense the bacterial peptidoglycan component, muramyl dipeptide. Upon 
      activation, Nod2 induces the production of various inflammatory molecules such as 
      cytokines and chemokines. Genetic linkage analysis identified and revealed three 
      major mutations in Nod2 that are associated with the development of Crohn's 
      disease. The objective of this study is to further characterize this protein by 
      determining whether Nod2 is posttranslationally modified by O-N-acetylglucosamine 
      (O-GlcNAc). O-GlcNAcylation is one type of posttranslational modification in 
      which the O-GlcNAc transferase transfers GlcNAc from UDP-GlcNAc to selected 
      serine and threonine residues of intracellular proteins. We found that wild-type 
      Nod2 and a Nod2 Crohn's-associated variant are O-GlcNAcylated and this 
      modification affects Nod2's ability to signal via the nuclear factor kappa B 
      pathway.
CI  - (c) The Author 2015. Published by Oxford University Press. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Hou, Ching-Wen
AU  - Hou CW
AD  - Department of Chemistry and Biochemistry.
FAU - Mohanan, Vishnu
AU  - Mohanan V
AD  - Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
FAU - Zachara, Natasha E
AU  - Zachara NE
AD  - Department of Biological Chemistry, The Johns Hopkins University School of 
      Medicine, Baltimore, MD 21205, USA.
FAU - Grimes, Catherine Leimkuhler
AU  - Grimes CL
AD  - Department of Chemistry and Biochemistry Department of Biological Sciences, 
      University of Delaware, Newark, DE 19716, USA cgrimes@udel.edu.
LA  - eng
GR  - P01 HL107153/HL/NHLBI NIH HHS/United States
GR  - P30 DK079637/DK/NIDDK NIH HHS/United States
GR  - P01HL107153/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150914
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (NF-kappa B)
RN  - 0 (NOD2 protein, human)
RN  - 0 (Nod2 Signaling Adaptor Protein)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/*metabolism
MH  - Glycosylation
MH  - HCT116 Cells
MH  - HEK293 Cells
MH  - Humans
MH  - Mutation
MH  - NF-kappa B/metabolism
MH  - Nod2 Signaling Adaptor Protein/genetics/*metabolism
MH  - *Protein Processing, Post-Translational
PMC - PMC4672147
OTO - NOTNLM
OT  - Crohn's disease
OT  - NF-kappaB
OT  - Nod2
OT  - O-GlcNAcylation
OT  - OGT
EDAT- 2015/09/16 06:00
MHDA- 2016/09/10 06:00
PMCR- 2017/01/01
CRDT- 2015/09/16 06:00
PHST- 2015/05/14 00:00 [received]
PHST- 2015/09/04 00:00 [accepted]
PHST- 2015/09/16 06:00 [entrez]
PHST- 2015/09/16 06:00 [pubmed]
PHST- 2016/09/10 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - cwv076 [pii]
AID - 10.1093/glycob/cwv076 [doi]
PST - ppublish
SO  - Glycobiology. 2016 Jan;26(1):13-8. doi: 10.1093/glycob/cwv076. Epub 2015 Sep 14.