PMID- 26373683
OWN - NLM
STAT- MEDLINE
DCOM- 20160609
LR  - 20181202
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 33
IP  - 6
DP  - 2015 Aug
TI  - Genome-wide screen of promoter methylation analysis of ES cells and ES derived 
      epidermal-like cells.
PG  - 398-406
LID - 10.1002/cbf.3129 [doi]
AB  - Embryonic stem cells (ESCs) are a population of pluripotent cells which can 
      differentiate into different cell types. However, there are few reports with 
      regard to differentiate ESCs into epidermal cells in vitro. In this study, we 
      aimed to investigate differentially methylated promoters involved in process of 
      differentiation from ESCs into epidermal-like cells (ELCs) induced by human 
      amnion. We successfully induced ESCs into ELCs, which expressed the surface 
      markers of CK19, CK15 and beta1-integrin. With MeDIP-chip arrays, we identified 3435 
      gene promoters to be differentially methylated, involving 894 HCP (high 
      CpG-containing promoter), 974 ICP (intermediate CpG-containing promoter) and 1567 
      LCP (low CpG-containing promoter) among all the 17,500 DNA methylation regions of 
      gene promoters in both ESCs and ELCs. Gene oncology and pathway analysis 
      demonstrated that these genes were involved in all the three categories of GO 
      enrichment analysis, including biological process, molecular function and 
      cellular component. All these data suggested that embryonic stem cells can 
      differentiate into epidermal-like cells and promoter methylation is of great 
      importance in this process.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Zhang, Ren-li
AU  - Zhang RL
AD  - Reproductive Medicine Center, Guangdong Academy of Medical Sciences/ Guangdong 
      General Hospital, Guangzhou, Guangdong, China.
FAU - Meng, Jin-xiu
AU  - Meng JX
AD  - Medical Research Center, Guangdong Academy of Medical Sciences/Guangdong General 
      Hospital, Guangzhou, Guangdong, China.
FAU - Liu, Cai-xia
AU  - Liu CX
AD  - Reproductive Medicine Center, Guangdong Academy of Medical Sciences/ Guangdong 
      General Hospital, Guangzhou, Guangdong, China.
FAU - Zhang, Li-li
AU  - Zhang LL
AD  - Reproductive Medicine Center, Guangdong Academy of Medical Sciences/ Guangdong 
      General Hospital, Guangzhou, Guangdong, China.
FAU - Han, Dong
AU  - Han D
AD  - Reproductive Medicine Center, Guangdong Academy of Medical Sciences/ Guangdong 
      General Hospital, Guangzhou, Guangdong, China.
FAU - Cai, Jia-jie
AU  - Cai JJ
AD  - Reproductive Medicine Center, Guangdong Academy of Medical Sciences/ Guangdong 
      General Hospital, Guangzhou, Guangdong, China.
FAU - Wen, An-min
AU  - Wen AM
AD  - Reproductive Medicine Center, Guangdong Academy of Medical Sciences/ Guangdong 
      General Hospital, Guangzhou, Guangdong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150916
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
SB  - IM
MH  - Amnion
MH  - Cell Differentiation
MH  - *DNA Methylation
MH  - Embryonic Stem Cells/cytology/*metabolism
MH  - Epidermal Cells
MH  - Epidermis/*metabolism
MH  - Epigenesis, Genetic
MH  - Genetic Testing/methods
MH  - *Genome, Human
MH  - Humans
MH  - *Promoter Regions, Genetic
OTO - NOTNLM
OT  - DNA methylation chip
OT  - embryonic stem cells
OT  - epidermal-like cells
OT  - immunoprecipitation of methylated DNA
OT  - promoter
EDAT- 2015/09/17 06:00
MHDA- 2016/06/10 06:00
CRDT- 2015/09/17 06:00
PHST- 2015/06/08 00:00 [received]
PHST- 2015/07/26 00:00 [revised]
PHST- 2015/08/03 00:00 [accepted]
PHST- 2015/09/17 06:00 [entrez]
PHST- 2015/09/17 06:00 [pubmed]
PHST- 2016/06/10 06:00 [medline]
AID - 10.1002/cbf.3129 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2015 Aug;33(6):398-406. doi: 10.1002/cbf.3129. Epub 2015 Sep 
      16.