PMID- 26373843
OWN - NLM
STAT- MEDLINE
DCOM- 20160824
LR  - 20151030
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 47
IP  - 11
DP  - 2015 Nov
TI  - Dendritic cell-based vaccination with lentiviral vectors encoding ubiquitinated 
      hepatitis B core antigen enhances hepatitis B virus-specific immune responses in 
      vivo.
PG  - 870-9
LID - 10.1093/abbs/gmv093 [doi]
AB  - The activity of hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) 
      plays a predominant role in the clearance of HBV. Dendritic cells (DCs) are key 
      antigen-presenting cells and play an important role in the initiation of immune 
      responses. We previously verified that lentiviral vector encoding ubiquitinated 
      hepatitis B core antigen (LV-Ub-HBcAg) effectively transduced DCs to induce 
      maturation, and the mature DCs efficiently induced T cell polarization to Th1 and 
      generated HBcAg-specific CTLs ex vivo. In this study, HBV-specific immune 
      responses of LV-Ub-HBcAg in BALB/c mice (H-2Kd) were evaluated. It was shown that 
      direct injection of LV-Ub-HBcAg increased the production of cytokines IL-2 and 
      IFN-gamma, elicited strong antibody responses, and remarkably generated a high 
      percentage of IFN-gamma+CD8+ T cells with HBV-specific CTL responses in BALB/c mice. 
      In addition, direct injection of LV-Ub-HBcAg induced potent anti-HBV immune 
      responses, similar to those elicited by in vitro-transduced DCs. In conclusion, 
      the DC-based therapeutic vaccine LV-Ub-HBcAg elicited specific antibody immune 
      responses and induced robust specific CTL activity in vivo.
CI  - (c) The Author 2015. Published by ABBS Editorial Office in association with Oxford 
      University Press on behalf of the Institute of Biochemistry and Cell Biology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
FAU - Dai, Shenglan
AU  - Dai S
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Zhuo, Meng
AU  - Zhuo M
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Song, Linlin
AU  - Song L
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Chen, Xiaohua
AU  - Chen X
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Yu, Yongsheng
AU  - Yu Y
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China.
FAU - Tang, Zhenghao
AU  - Tang Z
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China tzhhao@163.com zangguoqin@126.com.
FAU - Zang, Guoqing
AU  - Zang G
AD  - Department of Infectious Disease, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai 200233, China tzhhao@163.com zangguoqin@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150915
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Hepatitis B Core Antigens)
RN  - 0 (Hepatitis B Vaccines)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/*immunology
MH  - Genetic Vectors/administration & dosage
MH  - Hepatitis B Core Antigens/*chemistry/genetics/*immunology
MH  - Hepatitis B Vaccines/genetics/*immunology
MH  - Lentivirus/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes, Cytotoxic/immunology
OTO - NOTNLM
OT  - cytotoxic T lymphocyte
OT  - dendritic cell (DC)
OT  - hepatitis B virus (HBV)
OT  - lentiviral vector (LV)
OT  - ubiquitin
EDAT- 2015/09/17 06:00
MHDA- 2016/08/25 06:00
CRDT- 2015/09/17 06:00
PHST- 2015/05/08 00:00 [received]
PHST- 2015/06/30 00:00 [accepted]
PHST- 2015/09/17 06:00 [entrez]
PHST- 2015/09/17 06:00 [pubmed]
PHST- 2016/08/25 06:00 [medline]
AID - gmv093 [pii]
AID - 10.1093/abbs/gmv093 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2015 Nov;47(11):870-9. doi: 
      10.1093/abbs/gmv093. Epub 2015 Sep 15.