PMID- 26375414
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 2164-554X (Electronic)
IS  - 2164-5515 (Print)
IS  - 2164-5515 (Linking)
VI  - 12
IP  - 1
DP  - 2016
TI  - Neonatal vaccination with bacille Calmette-Guerin promotes the dendritic 
      development of hippocampal neurons.
PG  - 140-9
LID - 10.1080/21645515.2015.1056954 [doi]
AB  - Dendritic structure is sensitive to changes in the environment during brain 
      development. Accumulating evidence has demonstrated that early immune activation 
      can significantly affect neuronal development. Our study concentrated on the 
      morphological study of neural dendrites and spines in the hippocampal CA1 area 
      using Diolistic labeling with Sholl analysis and fractal analysis. The results 
      revealed that Bacille Calmette-Guerin (BCG) vaccination enhanced dendritic 
      complexity, as reflected by the increased number of intersections, number of 
      branch points and fractal dimension, and promoted neurite outgrowth. In addition, 
      BCG increased the density and promoted the maturation of dendritic spines. The 
      alterations in dendritic structure and spine morphology were observed at 2 and 
      4 w, but the differences were more apparent at 4 w than at 2 w. However, no 
      significant difference was observed at 8 w. Furthermore, we observed that BCG 
      increased the expression of hippocampal brain derived neurotrophic factor (BDNF) 
      and insulin-like growth factor 1 (IGF-1). Hippocampal BDNF/IGF-1 was positively 
      correlated with apical dendritic length, fractal dimension, and spine density. 
      Taken together, we show in this study that neonatal BCG vaccination promotes 
      dendritic development in developing hippocampal CA1 neurons, most likely by 
      increasing the expression of BDNF and IGF-1 in the hippocampus.
FAU - Li, Qingqing
AU  - Li Q
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
FAU - Zhang, Yuwei
AU  - Zhang Y
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
FAU - Zou, Juntao
AU  - Zou J
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
FAU - Qi, Fangfang
AU  - Qi F
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
FAU - Yang, Junhua
AU  - Yang J
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
FAU - Yuan, Qunfang
AU  - Yuan Q
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
FAU - Yao, Zhibin
AU  - Yao Z
AD  - a Department of Anatomy and Neurobiology ; Zhongshan School of Medicine; Sun 
      Yat-sen University ; Guangzhou , PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150916
PL  - United States
TA  - Hum Vaccin Immunother
JT  - Human vaccines & immunotherapeutics
JID - 101572652
RN  - 0 (BCG Vaccine)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - BCG Vaccine/*administration & dosage
MH  - Brain-Derived Neurotrophic Factor/biosynthesis
MH  - CA1 Region, Hippocampal/*cytology/*growth & development
MH  - Dendrites/*physiology
MH  - Gene Expression
MH  - Insulin-Like Growth Factor I/biosynthesis
MH  - Neurons/*physiology
MH  - Rats, Sprague-Dawley
PMC - PMC4962745
OTO - NOTNLM
OT  - dendritic complexity
OT  - fractal dimension
OT  - neurotrophin
OT  - sholl analysis
OT  - spine
EDAT- 2015/09/17 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/09/16
CRDT- 2015/09/17 06:00
PHST- 2015/09/17 06:00 [entrez]
PHST- 2015/09/17 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/09/16 00:00 [pmc-release]
AID - 1056954 [pii]
AID - 10.1080/21645515.2015.1056954 [doi]
PST - ppublish
SO  - Hum Vaccin Immunother. 2016;12(1):140-9. doi: 10.1080/21645515.2015.1056954. Epub 
      2015 Sep 16.