PMID- 26377571
OWN - NLM
STAT- MEDLINE
DCOM- 20160202
LR  - 20151023
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Linking)
VI  - 94
IP  - 11
DP  - 2015 Nov
TI  - RANTES and SDF-1 Are Keys in Cell-based Therapy of TMJ Osteoarthritis.
PG  - 1601-9
LID - 10.1177/0022034515604621 [doi]
AB  - The present study aimed to investigate the therapeutic effect of injections of 
      local bone marrow mesenchymal stem cells (BMSCs) on osteoarthritis (OA) of the 
      temporomandibular joint (TMJ) and to explore the role of stromal cell-derived 
      factor 1 (SDF-1) and regulated on activation, normal T-cell expressed and 
      secreted (RANTES) in this effect. Fundamentally, OA of the TMJ was induced by 
      unilateral anterior crossbite in mice. Exogenous green fluorescent 
      protein-labeled BMSCs (GFP-BMSCs) were weekly injected into the TMJ region for 4, 
      8, and 12 wk. The reparative effects of exogenous GFP-BMSCs were investigated by 
      morphological observation and micro-computed tomography. The differentiation of 
      GFP-BMSCs in the cartilage was examined by double immunofluorescence of GFPs with 
      type II collagen, and the expression of related factors in the condylar cartilage 
      was quantified by real-time polymerase chain reaction. The role of RANTES and 
      SDF-1 in the therapeutic effect of exogenous BMSCs was examined by both in vitro 
      and in vivo studies. The OA cartilage of the TMJ displays a synchronous increase 
      in SDF-1 and RANTES expression and a higher capability of attracting the 
      migration of GFP-BMSCs. The implanted GFP-BMSCs differentiated into type II 
      collagen-positive cells and reversed cartilage degradation and subchondral bone 
      loss in mice with OA of the TMJ. The migration of GFP-BMSCs towards OA cartilage 
      and the rescuing effect of GFP-BMSC injections were impaired by the inhibitors of 
      C-X-C chemokine receptor type 4 (CXCR4) and C-C chemokine receptor type 1 (CCR1), 
      which are the receptors of SDF-1 and RANTES, respectively. Our data indicated 
      that SDF-1/CXCR4 and RANTES/CCR1 signals are pivotal and function synergistically 
      in the recruitment of GFP-BMSCs towards degraded cartilage in mice OA of the TMJ.
CI  - (c) International & American Associations for Dental Research 2015.
FAU - Lu, L
AU  - Lu L
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Stomatology, Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Zhang, M
AU  - Zhang M
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China.
FAU - Zhang, H
AU  - Zhang H
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China.
FAU - Liao, L
AU  - Liao L
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China.
FAU - Yang, T
AU  - Yang T
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China.
FAU - Zhang, J
AU  - Zhang J
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China.
FAU - Xian, L
AU  - Xian L
AD  - Department of Hematology, Johns Hopkins University, Baltimore, MD, USA.
FAU - Chen, D
AU  - Chen D
AD  - Department of Biochemistry, Rush University Medical Center, Chicago, IL, USA.
FAU - Wang, M
AU  - Wang M
AD  - State Key Laboratory of Military Stomatology, Department of Oral Anatomy and 
      Physiology and TMD, School of Stomatology, Fourth Military Medical University, 
      Xi'an, China mqwang@fmmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150916
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Ccl5 protein, mouse)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Collagen Type II)
RN  - 0 (Cxcl12 protein, mouse)
SB  - IM
MH  - Animals
MH  - Chemokine CCL5/metabolism/*physiology
MH  - Chemokine CXCL12/metabolism/*physiology
MH  - Collagen Type II/physiology
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoarthritis/*therapy
MH  - Real-Time Polymerase Chain Reaction
MH  - Temporomandibular Joint Disorders/*therapy
MH  - Treatment Outcome
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - cartilage
OT  - chemokines
OT  - dental occlusion
OT  - mesenchymal stem cells
OT  - regeneration
OT  - temporomandibular joint
EDAT- 2015/09/18 06:00
MHDA- 2016/02/03 06:00
CRDT- 2015/09/18 06:00
PHST- 2015/09/18 06:00 [entrez]
PHST- 2015/09/18 06:00 [pubmed]
PHST- 2016/02/03 06:00 [medline]
AID - 0022034515604621 [pii]
AID - 10.1177/0022034515604621 [doi]
PST - ppublish
SO  - J Dent Res. 2015 Nov;94(11):1601-9. doi: 10.1177/0022034515604621. Epub 2015 Sep 
      16.