PMID- 26378973
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20191210
IS  - 1557-7759 (Electronic)
IS  - 1530-3667 (Linking)
VI  - 15
IP  - 9
DP  - 2015 Sep
TI  - Development of an Orientia tsutsugamushi Lc-1 Murine Intraperitoneal Challenge 
      Model for Scrub Typhus: Determination of Murine Lethal Dose (MuLD50), Tissue 
      Bacterial Loads, and Clinical Outcomes.
PG  - 539-44
LID - 10.1089/vbz.2015.1773 [doi]
AB  - Currently, no vaccine has been developed to protect humans from naturally 
      acquired heterologous Orientia tsutsugamushi infections. To enhance the validity 
      of vaccine candidates, we are developing a murine chigger challenge model with 
      the O. tsutsugamushi Lc-1-infected Leptotrombidium chiangraiensis Line-1. To this 
      end, an intraperitoneal (i.p.) murine challenge model using an O. tsutsugamushi 
      Lc-1 isolate was developed for eventual validation of the chigger challenge 
      model. We have determined that the murine lethal dose that kills 50% of the 
      challenged mice (MuLD50) of a liver/spleen homogenate developed from O. 
      tsutsugamushi Lc-1-infected ICR Swiss mice to be 10(-6.9). Employing different 
      inoculum doses of this homogenate, the bacterial load using quantitative 
      real-time PCR (qPCR) was determined to range from 60 to 1.6 x 10(5) genome 
      equivalent copies (GEC)/muL of liver and 33.4 to 2.2 x 10(5) GEC/muL of spleen 
      tissue. The clinical outcomes relative to homogenate dose levels followed a 
      dose-dependent pattern. The successful development and characterization of the O. 
      tsutsugamushi Lc-1 i.p. challenge model will assist in the development and 
      validation of a mouse chigger challenge scrub typhus model.
FAU - Lurchachaiwong, Woradee
AU  - Lurchachaiwong W
AD  - 1 Department of Entomology, US Army Medical Component, Armed Forces Research 
      Institute of Medical Sciences , Bangkok, Thailand .
FAU - McCardle, Wesley
AU  - McCardle W
AD  - 1 Department of Entomology, US Army Medical Component, Armed Forces Research 
      Institute of Medical Sciences , Bangkok, Thailand .
FAU - Chan, Teik-Chye
AU  - Chan TC
AD  - 2 Viral and Rickettsial Diseases Department, Naval Medical Research Center , 
      Silver Spring, Maryland.
FAU - Schuster, Anthony L
AU  - Schuster AL
AD  - 1 Department of Entomology, US Army Medical Component, Armed Forces Research 
      Institute of Medical Sciences , Bangkok, Thailand .
FAU - Richards, Allen L
AU  - Richards AL
AD  - 2 Viral and Rickettsial Diseases Department, Naval Medical Research Center , 
      Silver Spring, Maryland.
AD  - 3 Preventive Medicine and Biometrics Department, Uniformed Services University of 
      the Health Sciences , Bethesda, Maryland.
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - Vector Borne Zoonotic Dis
JT  - Vector borne and zoonotic diseases (Larchmont, N.Y.)
JID - 100965525
SB  - IM
MH  - Animals
MH  - Bacterial Load
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Injections, Intraperitoneal
MH  - Lethal Dose 50
MH  - Liver/microbiology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Orientia tsutsugamushi/genetics/*physiology
MH  - Scrub Typhus/*microbiology
MH  - Spleen/microbiology
MH  - Trombiculidae/*microbiology
OTO - NOTNLM
OT  - Chigger mite
OT  - Modeling
OT  - Rickettsia
OT  - Scrub typhus
OT  - Tsutsugamushi
EDAT- 2015/09/18 06:00
MHDA- 2017/04/18 06:00
CRDT- 2015/09/18 06:00
PHST- 2015/09/18 06:00 [entrez]
PHST- 2015/09/18 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
AID - 10.1089/vbz.2015.1773 [doi]
PST - ppublish
SO  - Vector Borne Zoonotic Dis. 2015 Sep;15(9):539-44. doi: 10.1089/vbz.2015.1773.