PMID- 26379775
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150918
LR  - 20201001
IS  - 1754-6834 (Print)
IS  - 1754-6834 (Electronic)
IS  - 1754-6834 (Linking)
VI  - 8
DP  - 2015
TI  - Metabolic engineering of Escherichia coli for production of 
      (2S,3S)-butane-2,3-diol from glucose.
PG  - 143
LID - 10.1186/s13068-015-0324-x [doi]
LID - 143
AB  - BACKGROUND: Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with 
      various industrial applications. 2,3-BD exists in three stereoisomeric forms: 
      (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes 
      have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production. RESULTS: The 
      production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of 
      recombinant Escherichia coli coexpressing the alpha-acetolactate synthase and 
      meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens 
      strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 
      (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions 
      were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, 
      (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric 
      purity of 95.0 % using the metabolically engineered strain from glucose. 
      CONCLUSIONS: The engineered E. coli strain is the first one that can be used in 
      the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated 
      that the method developed here would be a promising process for efficient 
      (2S,3S)-2,3-BD production.
FAU - Chu, Haipei
AU  - Chu H
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
FAU - Xin, Bo
AU  - Xin B
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
FAU - Liu, Peihai
AU  - Liu P
AD  - Rizhao Entry-Exit Inspection and Quarantine Bureau, Rizhao, 276800 People's 
      Republic of China.
FAU - Wang, Yu
AU  - Wang Y
AD  - State Key Laboratory of Microbial Metabolism, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 People's Republic 
      of China.
FAU - Li, Lixiang
AU  - Li L
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
FAU - Liu, Xiuxiu
AU  - Liu X
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
FAU - Ma, Cuiqing
AU  - Ma C
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
FAU - Xu, Ping
AU  - Xu P
AD  - State Key Laboratory of Microbial Metabolism, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 People's Republic 
      of China.
FAU - Gao, Chao
AU  - Gao C
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100 
      People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20150915
PL  - England
TA  - Biotechnol Biofuels
JT  - Biotechnology for biofuels
JID - 101316935
PMC - PMC4570510
OTO - NOTNLM
OT  - (2S,3S)-Butane-2,3-diol
OT  - Metabolic engineering
OT  - meso-Butane-2,3-diol dehydrogenase
OT  - alpha-Acetolactate synthase
EDAT- 2015/09/18 06:00
MHDA- 2015/09/18 06:01
PMCR- 2015/09/15
CRDT- 2015/09/18 06:00
PHST- 2015/06/22 00:00 [received]
PHST- 2015/08/25 00:00 [accepted]
PHST- 2015/09/18 06:00 [entrez]
PHST- 2015/09/18 06:00 [pubmed]
PHST- 2015/09/18 06:01 [medline]
PHST- 2015/09/15 00:00 [pmc-release]
AID - 324 [pii]
AID - 10.1186/s13068-015-0324-x [doi]
PST - epublish
SO  - Biotechnol Biofuels. 2015 Sep 15;8:143. doi: 10.1186/s13068-015-0324-x. 
      eCollection 2015.