PMID- 26381135
OWN - NLM
STAT- MEDLINE
DCOM- 20160912
LR  - 20220317
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 14
IP  - 11
DP  - 2015 Nov 6
TI  - Highly Pathogenic H5N1 and Novel H7N9 Influenza A Viruses Induce More Profound 
      Proteomic Host Responses than Seasonal and Pandemic H1N1 Strains.
PG  - 4511-23
LID - 10.1021/acs.jproteome.5b00196 [doi]
AB  - Influenza A viruses (IAV) are important human and animal pathogens with potential 
      for causing pandemics. IAVs exhibit a wide spectrum of clinical illness in 
      humans, from relatively mild infections by seasonal strains to acute respiratory 
      distress syndrome during infections with some highly pathogenic avian influenza 
      (HPAI) viruses. In the present study, we infected A549 human cells with seasonal 
      H1N1 (sH1N1), 2009 pandemic H1N1 (pdmH1N1), or novel H7N9 and HPAI H5N1 strains. 
      We used multiplexed isobaric tags for relative and absolute quantification to 
      measure proteomic host responses to these different strains at 1, 3, and 6 h 
      post-infection. Our analyses revealed that both H7N9 and H5N1 strains induced 
      more profound changes to the A549 global proteome compared to those with 
      low-pathogenicity H1N1 virus infection, which correlates with the higher 
      pathogenicity these strains exhibit at the organismal level. Bioinformatics 
      analysis revealed important modulation of the nuclear factor erythroid 2-related 
      factor 2 (NRF2) oxidative stress response in infection. Cellular fractionation 
      and Western blotting suggested that the phosphorylated form of NRF2 is not 
      imported to the nucleus in H5N1 and H7N9 virus infections. Fibronectin was also 
      strongly inhibited in infection with H5N1 and H7N9 strains. This is the first 
      known comparative proteomic study of the host response to H7N9, H5N1, and H1N1 
      viruses and the first time NRF2 is shown to be implicated in infection with 
      highly pathogenic strains of influenza.
FAU - Simon, Philippe Francois
AU  - Simon PF
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba , Winnipeg, Manitoba, R3E 0J9 Canada.
FAU - McCorrister, Stuart
AU  - McCorrister S
FAU - Hu, Pingzhao
AU  - Hu P
AD  - Department of Biochemistry and Medical Genetics, University of Manitoba , 
      Winnipeg, Manitoba, R3T 2N2 Canada.
FAU - Chong, Patrick
AU  - Chong P
FAU - Silaghi, Alex
AU  - Silaghi A
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba , Winnipeg, Manitoba, R3E 0J9 Canada.
FAU - Westmacott, Garrett
AU  - Westmacott G
FAU - Coombs, Kevin M
AU  - Coombs KM
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba , Winnipeg, Manitoba, R3E 0J9 Canada.
AD  - Manitoba Center for Proteomics and Systems Biology, University of Manitoba , 
      Winnipeg, Manitoba, R3E 3P4 Canada.
FAU - Kobasa, Darwyn
AU  - Kobasa D
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba , Winnipeg, Manitoba, R3E 0J9 Canada.
LA  - eng
GR  - MOP-106713/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151009
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Fibronectins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Proteome)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism/virology
MH  - Computational Biology/methods
MH  - Cytosol/metabolism/virology
MH  - Epithelial Cells/*metabolism/virology
MH  - Fibronectins/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Influenza A Virus, H1N1 Subtype/*physiology
MH  - Influenza A Virus, H5N1 Subtype/*pathogenicity/physiology
MH  - Influenza A Virus, H7N9 Subtype/*pathogenicity/physiology
MH  - NF-E2-Related Factor 2/*genetics/metabolism
MH  - Oxidative Stress
MH  - Phosphorylation
MH  - Protein Transport
MH  - Proteome/*genetics/metabolism
MH  - Respiratory Mucosa/metabolism/virology
MH  - Signal Transduction
MH  - Virulence
OTO - NOTNLM
OT  - FN1
OT  - H1N1
OT  - H5N1
OT  - H7N9
OT  - NRF2
OT  - avian influenza
OT  - fibronectin
OT  - oxidative stress
EDAT- 2015/09/19 06:00
MHDA- 2016/09/13 06:00
CRDT- 2015/09/19 06:00
PHST- 2015/09/19 06:00 [entrez]
PHST- 2015/09/19 06:00 [pubmed]
PHST- 2016/09/13 06:00 [medline]
AID - 10.1021/acs.jproteome.5b00196 [doi]
PST - ppublish
SO  - J Proteome Res. 2015 Nov 6;14(11):4511-23. doi: 10.1021/acs.jproteome.5b00196. 
      Epub 2015 Oct 9.