PMID- 26381618
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20211203
IS  - 1422-6421 (Electronic)
IS  - 1422-6405 (Print)
IS  - 1422-6405 (Linking)
VI  - 200
IP  - 6
DP  - 2015
TI  - Fluoride Modulates Parathyroid Hormone Secretion in vivo and in vitro.
PG  - 413-23
LID - 10.1159/000438699 [doi]
AB  - The study objective was to investigate the effects of fluoride on intact 
      parathyroid hormone (iPTH) secretion. Thyro-parathyroid complexes (TPC) from C3H 
      (n = 18) and B6 (n = 18) mice were cultured in Ca(2)(+)-optimized medium. TPC were 
      treated with 0, 250, or 500 microM NaF for 24 h and secreted iPTH assayed by ELISA. 
      C3H (n = 78) and B6 (n = 78) mice were gavaged once with distilled or fluoride 
      (0.001 mg [F(-)]/g of body weight) water. At serial time points (0.5-96 h) serum 
      iPTH, fluoride, total calcium, phosphorus, and magnesium levels were determined. 
      Expression of genes involved in mineral regulation via the 
      bone-parathyroid-kidney (BPK) axis, such as parathyroid hormone (Pth), 
      calcium-sensing receptor (Casr), vitamin D receptor (Vdr), parathyroid 
      hormone-like hormone (Pthlh), fibroblast growth factor 23 (Fgf23), alpha-Klotho 
      (alphaKlotho), fibroblast growth factor receptor 1c (Fgf1rc), tumor necrosis factor 
      11 (Tnfs11), parathyroid hormone receptor 1 (Pth1r), solute carrier family 34 
      member 1 (Slc34a1), solute carrier 9 member 3 regulator 1 (Slc9a3r1), chloride 
      channel 5 (Clcn5), and PDZ domain-containing 1 (Pdzk1), was determined in TPC, 
      humeri, and kidneys at 24 h. An in vitro decrease in iPTH was seen in C3H and B6 
      TPC at 500 microM (p < 0.001). In vivo levels of serum fluoride peaked at 0.5 h in 
      both C3H (p = 0.002) and B6 (p = 0.01). In C3H, iPTH decreased at 24 h (p < 
      0.0001), returning to baseline at 48 h. In B6, iPTH increased at 12 h (p < 
      0.001), returning to baseline at 24 h. Serum total calcium, phosphorus, and 
      magnesium levels did not change significantly. Pth, Casr,alphaKlotho,Fgf1rc,Vdr, and 
      Pthlh were significantly upregulated in C3H TPC compared to B6. In conclusion, 
      the effects of fluoride on TPC in vitro were equivalent between the 2 mouse 
      strains. However, fluoride demonstrated an early strain-dependent effect on iPTH 
      secretion in vivo. Both strains demonstrated differences in the expression of 
      genes involved in the BPK axis, suggesting a possible role in the physiologic 
      handling of fluoride.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Puranik, Chaitanya P
AU  - Puranik CP
AD  - Oral Biology PhD Curriculum, Gillings School of Global Public Health, University 
      of North Carolina at Chapel Hill, Chapel Hill, N.C., USA.
FAU - Ryan, Kathleen A
AU  - Ryan KA
FAU - Yin, Zhaoyu
AU  - Yin Z
FAU - Martinez-Mier, E Angeles
AU  - Martinez-Mier EA
FAU - Preisser, John S
AU  - Preisser JS
FAU - Everett, Eric T
AU  - Everett ET
LA  - eng
GR  - R01 DE018104/DE/NIDCR NIH HHS/United States
GR  - R01DE018104/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150919
PL  - Switzerland
TA  - Cells Tissues Organs
JT  - Cells, tissues, organs
JID - 100883360
RN  - 0 (Fgf23 protein, mouse)
RN  - 0 (Parathyroid Hormone)
RN  - 27YLU75U4W (Phosphorus)
RN  - 7Q7P4S7RRE (Fibroblast Growth Factor-23)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - I38ZP9992A (Magnesium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/blood
MH  - Cells, Cultured
MH  - Fibroblast Growth Factor-23
MH  - Gene Expression Regulation/drug effects
MH  - Magnesium/blood
MH  - Male
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Parathyroid Glands/cytology/drug effects/metabolism
MH  - Parathyroid Hormone/*blood/metabolism
MH  - Phosphorus/blood
MH  - Sodium Fluoride/administration & dosage/blood/*pharmacology
PMC - PMC4679577
MID - NIHMS708704
COIS- Conflicts of interest The authors do not have any conflicts of interest.
EDAT- 2015/09/19 06:00
MHDA- 2016/10/07 06:00
PMCR- 2016/12/01
CRDT- 2015/09/19 06:00
PHST- 2015/07/14 00:00 [accepted]
PHST- 2015/09/19 06:00 [entrez]
PHST- 2015/09/19 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
PHST- 2016/12/01 00:00 [pmc-release]
AID - 000438699 [pii]
AID - 10.1159/000438699 [doi]
PST - ppublish
SO  - Cells Tissues Organs. 2015;200(6):413-23. doi: 10.1159/000438699. Epub 2015 Sep 
      19.