PMID- 26382064
OWN - NLM
STAT- MEDLINE
DCOM- 20180507
LR  - 20181202
IS  - 1932-8745 (Electronic)
IS  - 0161-0457 (Linking)
VI  - 38
IP  - 4
DP  - 2016 Jul
TI  - Chemical analysis and biological properties of two different formulations of 
      white portland cements.
PG  - 303-16
LID - 10.1002/sca.21270 [doi]
AB  - White Portland cement (WPC) has generated research interests in the field of 
      endodontics. This study compared between the properties of two formulations of 
      white Portland cement (WPC) of different origin (Malaysia [MA] and Egypt [EG]). 
      WPCs with and without calcium chloride dihydrate were prepared. Scanning electron 
      microscope (SEM), energy dispersive X-ray micro-analysis, and X-ray diffraction 
      were used for surface morphology evaluation, elemental, and phase analysis, 
      respectively. After the preparation of optimized serial dilutions, the 
      cytotoxicity was evaluated on human periodontal ligament fibroblasts (HPLFs) and 
      dental pulp stem cells (DPSCs) using methyl-thiazol-diphenyltetrazolium assay 
      after 24 and 72 h. Cell attachment properties were examined under SEM after 24 
      and 72 h. Results showed that the surface morphology and chemical composition of 
      both formulations demonstrated detectable variations. The cytotoxicity evaluation 
      showed different cellular responses of HPLFs compared to DSPCs. Both formulations 
      favored the viability of HPLFs. However, the fast set formulations demonstrated 
      severe cytotoxicity on DPSCs. Significant differences between EGWPC and MAWPC 
      were identified (p < 0.05). The cell attachment properties were favorable; 
      however, HPLFs attached and spread over the samples better than DPSCs. In 
      conclusion, WPC of different origin may show differences in chemical and 
      biological properties. The addition of CaCl2 .2H2 O to WPC can affect its 
      properties. Human cell types may react differently towards different formulations 
      of WPCs. SCANNING 38:303-316, 2016. (c) 2015 Wiley Periodicals, Inc.
CI  - (c) Wiley Periodicals, Inc.
FAU - Ahmed, Hany Mohamed Aly
AU  - Ahmed HM
AD  - School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 
      Malaysia.
FAU - Luddin, Norhayati
AU  - Luddin N
AD  - School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 
      Malaysia.
FAU - Kannan, Thirumulu Ponnuraj
AU  - Kannan TP
AD  - School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 
      Malaysia.
AD  - Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 
      Kubang Kerian, Kelantan, Malaysia.
FAU - Mokhtar, Khairani Idah
AU  - Mokhtar KI
AD  - School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 
      Malaysia.
AD  - Kulliyyah of Dentistry, International Islamic University Malaysia, Bandar Indera 
      Mahkota, Pahang, Kuantan, Malaysia.
FAU - Ahmad, Azlina
AU  - Ahmad A
AD  - School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, 
      Malaysia.
AD  - Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 
      Kubang Kerian, Kelantan, Malaysia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150918
PL  - England
TA  - Scanning
JT  - Scanning
JID - 7903371
RN  - 0 (Calcium Compounds)
RN  - 0 (Root Canal Filling Materials)
RN  - 0 (Silicates)
SB  - IM
MH  - *Calcium Compounds
MH  - Humans
MH  - Materials Testing
MH  - *Root Canal Filling Materials
MH  - *Silicates
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - Egypt
OT  - Malaysia
OT  - calcium chloride dihydrate
OT  - cell attachment
OT  - chemical analysis
OT  - cytotoxicity
OT  - dental pulp stem cells
OT  - human periodontal ligament fibroblasts
OT  - white Portland cement
EDAT- 2015/09/19 06:00
MHDA- 2018/05/08 06:00
CRDT- 2015/09/19 06:00
PHST- 2015/07/20 00:00 [received]
PHST- 2015/08/28 00:00 [revised]
PHST- 2015/09/01 00:00 [accepted]
PHST- 2015/09/19 06:00 [entrez]
PHST- 2015/09/19 06:00 [pubmed]
PHST- 2018/05/08 06:00 [medline]
AID - 10.1002/sca.21270 [doi]
PST - ppublish
SO  - Scanning. 2016 Jul;38(4):303-16. doi: 10.1002/sca.21270. Epub 2015 Sep 18.