PMID- 26384166
OWN - NLM
STAT- MEDLINE
DCOM- 20160223
LR  - 20151117
IS  - 1879-1891 (Electronic)
IS  - 0002-9394 (Linking)
VI  - 160
IP  - 6
DP  - 2015 Dec
TI  - beta-Amyloid Precursor Protein and Ubiquitin Immunohistochemistry Aid in the 
      Evaluation of Infant Autopsy Eyes With Abusive Head Trauma.
PG  - 1285-1295.e6
LID - S0002-9394(15)00557-7 [pii]
LID - 10.1016/j.ajo.2015.09.005 [doi]
AB  - PURPOSE: To investigate beta-amyloid precursor protein (beta-APP), ubiquitin, and glial 
      fibrillary acid protein (GFAP) immunostaining as a diagnostic tool to aid in the 
      discrimination between abusive head trauma and nonabusive head trauma in 
      postmortem ocular histopathologic investigation. DESIGN: Retrospective 
      cross-sectional study. METHODS: Seventy-four eyes of 37 infants were studied for 
      hemorrhage and immunohistochemical expression of beta-APP, ubiquitin, and GFAP in 
      the retina and optic nerve. Infants were assigned to abusive head trauma or 
      control groups, according to published criteria. RESULTS: In the abusive head 
      trauma group, positive beta-APP and ubiquitin immunostaining of the retina was 
      significantly more likely to be found than in the control group, odds ratio (OR) 
      11.4, confidence interval (CI) 2.9-44.3; P < .001 and OR 8.8, CI 2.2-34.5; 
      P = .002, respectively. Positive correlations were found between retinal 
      expression of beta-APP and ubiquitin immunostaining and retinal hemorrhage. Vitreal 
      hemorrhages, orbital fat hemorrhages, and macular folds could only be identified 
      in abusive head trauma cases. Retinal hemorrhages were significantly more severe, 
      occupied a larger proportion of the retina, and involved more retinal layers in 
      abusive head trauma compared to controls (OR 2.7, CI 1.7-4.4; P < .001). 
      CONCLUSIONS: This study shows correlations between positive retinal beta-APP and 
      ubiquitin immunostaining as a sign of axonal injury in abusive head trauma. 
      Axonal injury is a useful pathologic feature that can be demonstrated in 
      postmortem ocular investigation of deceased children using immunohistochemical 
      staining for beta-APP and ubiquitin with a high OR for abusive head trauma when 
      compared to controls.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Bais, Babette
AU  - Bais B
AD  - Department of Pathology, Section Ophthalmic Pathology, ErasmusMC University 
      Medical Center Rotterdam, Rotterdam, Netherlands; Department of Forensic Medicine 
      of the Netherlands Forensic Institute, The Hague, Netherlands.
FAU - Kubat, Bela
AU  - Kubat B
AD  - Department of Forensic Medicine of the Netherlands Forensic Institute, The Hague, 
      Netherlands; Department of Pathology, Maastricht University Medical Center, 
      Maastricht, Netherlands.
FAU - Motazedi, Ehsan
AU  - Motazedi E
AD  - Department of Biostatistics, ErasmusMC University Medical Center Rotterdam, 
      Rotterdam, Netherlands.
FAU - Verdijk, Robert M
AU  - Verdijk RM
AD  - Department of Pathology, Section Ophthalmic Pathology, ErasmusMC University 
      Medical Center Rotterdam, Rotterdam, Netherlands. Electronic address: 
      r.verdijk@erasmusmc.nl.
LA  - eng
PT  - Journal Article
DEP - 20150914
PL  - United States
TA  - Am J Ophthalmol
JT  - American journal of ophthalmology
JID - 0370500
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Ubiquitin)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*analysis
MH  - Autopsy
MH  - *Child Abuse
MH  - Craniocerebral Trauma/*complications/diagnosis
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Retinal Hemorrhage/*diagnosis/etiology/metabolism
MH  - Retrospective Studies
MH  - Ubiquitin/*analysis
EDAT- 2015/09/19 06:00
MHDA- 2016/02/26 06:00
CRDT- 2015/09/19 06:00
PHST- 2015/05/08 00:00 [received]
PHST- 2015/09/03 00:00 [revised]
PHST- 2015/09/04 00:00 [accepted]
PHST- 2015/09/19 06:00 [entrez]
PHST- 2015/09/19 06:00 [pubmed]
PHST- 2016/02/26 06:00 [medline]
AID - S0002-9394(15)00557-7 [pii]
AID - 10.1016/j.ajo.2015.09.005 [doi]
PST - ppublish
SO  - Am J Ophthalmol. 2015 Dec;160(6):1285-1295.e6. doi: 10.1016/j.ajo.2015.09.005. 
      Epub 2015 Sep 14.