PMID- 26384586
OWN - NLM
STAT- MEDLINE
DCOM- 20160722
LR  - 20220316
IS  - 1755-5949 (Electronic)
IS  - 1755-5930 (Print)
IS  - 1755-5930 (Linking)
VI  - 21
IP  - 11
DP  - 2015 Nov
TI  - Bone Marrow Endothelial Progenitor Cell Transplantation After Ischemic Stroke: An 
      Investigation Into Its Possible Mechanism.
PG  - 877-86
LID - 10.1111/cns.12447 [doi]
AB  - AIMS: We tested the hypothesis that endothelial progenitor cell (EPC)-mediated 
      functional recovery after stroke may be associated with the endothelial nitric 
      oxide synthase (eNOS)/brain-derived neurotrophic factor (BDNF) signaling pathway. 
      METHODS: Mice were infused with either EPCs or saline after being subjected to 
      middle cerebral artery occlusion. The EPC-treated mice also received intravenous 
      injections of either Nomega-nitro-l-arginine methyl ester (L-NAME, the NOS inhibitor) 
      or saline. RESULTS: The activation of eNOS and the expression of BDNF were 
      significantly increased in ischemic brain of the EPC-treated mice, along with 
      increased angiogenesis and neurogenesis. On diffusion tensor imaging (DTI), 
      significant increases in fractional anisotropy and fiber count were observed in 
      white matter, indicating axonal growth stimulated by EPCs. However, the 
      EPC-treated mice that were received an L-NAME injection failed to exhibit the 
      observed increases in angiogenesis, neurogenesis, and axonal growth. In addition, 
      the neurons cocultured with EPCs in vitro exhibited the increased expression of 
      BDNF and decreased apoptosis after oxygen-glucose deprivation compared with the 
      control group. This EPC-induced protective effect was virtually absent in the 
      L-NAME treatment group. CONCLUSION: The eNOS/BDNF pathway may be involved in the 
      EPC-mediated functional recovery of stroke mice. DTI is feasible for dynamically 
      tracking the orientation of axonal projections after EPC treatment.
CI  - (c) 2015 John Wiley & Sons Ltd.
FAU - Bai, Ying-Ying
AU  - Bai YY
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Peng, Xin-Gui
AU  - Peng XG
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Wang, Li-Shan
AU  - Wang LS
AD  - Department of General Surgery, Zhongda Hospital, School of Medicine, Southeast 
      University, Nanjing, China.
FAU - Li, Zi-Hui
AU  - Li ZH
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Wang, Yuan-Cheng
AU  - Wang YC
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Lu, Chun-Qiang
AU  - Lu CQ
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Ding, Jie
AU  - Ding J
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Li, Pei-Cheng
AU  - Li PC
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Zhao, Zhen
AU  - Zhao Z
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
FAU - Ju, Sheng-Hong
AU  - Ju SH
AD  - Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of 
      Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150919
PL  - England
TA  - CNS Neurosci Ther
JT  - CNS neuroscience & therapeutics
JID - 101473265
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Disease Models, Animal
MH  - Endothelial Progenitor Cells/*physiology/*transplantation
MH  - Enzyme Inhibitors/therapeutic use
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Infarction, Middle Cerebral Artery/complications/drug therapy/*surgery
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NG-Nitroarginine Methyl Ester/therapeutic use
MH  - Neovascularization, Pathologic/etiology/therapy
MH  - Nervous System Diseases/etiology
MH  - Neurogenesis/drug effects/physiology
MH  - Neurons/drug effects
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Time Factors
PMC - PMC6493116
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Diffusion tensor imaging
OT  - Endothelial nitric oxide synthase
OT  - Endothelial progenitor cells
OT  - Ischemic stroke
COIS- The authors declare no conflict of interest.
EDAT- 2015/09/20 06:00
MHDA- 2016/07/23 06:00
PMCR- 2015/09/19
CRDT- 2015/09/20 06:00
PHST- 2014/10/17 00:00 [received]
PHST- 2015/08/09 00:00 [revised]
PHST- 2015/08/10 00:00 [accepted]
PHST- 2015/09/20 06:00 [entrez]
PHST- 2015/09/20 06:00 [pubmed]
PHST- 2016/07/23 06:00 [medline]
PHST- 2015/09/19 00:00 [pmc-release]
AID - CNS12447 [pii]
AID - 10.1111/cns.12447 [doi]
PST - ppublish
SO  - CNS Neurosci Ther. 2015 Nov;21(11):877-86. doi: 10.1111/cns.12447. Epub 2015 Sep 
      19.