PMID- 26388293
OWN - NLM
STAT- MEDLINE
DCOM- 20160818
LR  - 20151029
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 27
IP  - 11
DP  - 2015 Nov
TI  - Involvement of Endogenous Brain-Derived Neurotrophic Factor in 
      Hypothalamic-Pituitary-Adrenal Axis Activity.
PG  - 850-60
LID - 10.1111/jne.12324 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) appears to be highly involved in 
      hypothalamic-pituitary-adrenal (HPA) axis regulation during adulthood, playing an 
      important role in homeostasis maintenance. The present study aimed to determine 
      the involvement of BDNF in HPA axis activity under basal and stress conditions 
      via partial inhibition of this endogenous neurotrophin. Experiments were 
      conducted in rats and mice with two complementary approaches: (i) BDNF knockdown 
      with stereotaxic delivery of BDNF-specific small interfering RNA (siRNA) into the 
      lateral ventricle of adult male rats and (ii) genetically induced knockdown (KD) 
      of BDNF expression specifically in the central nervous system during the first 
      ontogenesis in mice (KD mice). Delivery of siRNA in the rat brain decreased BDNF 
      levels in the hippocampus (-31%) and hypothalamus (-35%) but not in the amygdala, 
      frontal cortex and pituitary. In addition, siRNA induced no change of the basal 
      HPA axis activity. BDNF siRNA rats exhibited decreased BDNF levels and 
      concomitant altered adrenocortoctrophic hormone (ACTH) and corticosterone 
      responses to restraint stress, suggesting the involvement of BDNF in the HPA axis 
      adaptive response to stress. In KD mice, BDNF levels in the hippocampus and 
      hypothalamus were decreased by 20% in heterozygous and by 60% in homozygous 
      animals compared to wild-type littermates. Although, in heterozygous KD mice, no 
      significant change was observed in the basal levels of plasma ACTH and 
      corticosterone, both hormones were significantly increased in homozygous KD mice, 
      demonstrating that robust cerebral BDNF inhibition (60%) is necessary to affect 
      basal HPA axis activity. All of these results in both rats and mice demonstrate 
      the involvement and importance of a robust endogenous pool of BDNF in basal HPA 
      axis regulation and the pivotal function of de novo BDNF synthesis in the 
      establishment of an adapted response to stress.
CI  - (c) 2015 British Society for Neuroendocrinology.
FAU - Naert, G
AU  - Naert G
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, Inserm, U1198 
      Montpellier, France.
AD  - University of Montpellier, Montpellier, France.
AD  - EPHE, Paris, France.
FAU - Zussy, C
AU  - Zussy C
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, Inserm, U1198 
      Montpellier, France.
AD  - University of Montpellier, Montpellier, France.
AD  - EPHE, Paris, France.
FAU - Tran Van Ba, C
AU  - Tran Van Ba C
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, Inserm, U1198 
      Montpellier, France.
AD  - University of Montpellier, Montpellier, France.
AD  - EPHE, Paris, France.
FAU - Chevallier, N
AU  - Chevallier N
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, Inserm, U1198 
      Montpellier, France.
AD  - University of Montpellier, Montpellier, France.
AD  - EPHE, Paris, France.
FAU - Tang, Y-P
AU  - Tang YP
AD  - Department of Cell Biology and Anatomy, Louisiana State University Health 
      Sciences Center (LSUHSC), New Orleans, LA, USA.
FAU - Maurice, T
AU  - Maurice T
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, Inserm, U1198 
      Montpellier, France.
AD  - University of Montpellier, Montpellier, France.
AD  - EPHE, Paris, France.
FAU - Givalois, L
AU  - Givalois L
AD  - Molecular Mechanisms in Neurodegenerative Dementia Laboratory, Inserm, U1198 
      Montpellier, France.
AD  - University of Montpellier, Montpellier, France.
AD  - EPHE, Paris, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Small Interfering)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Adrenocorticotropic Hormone/blood
MH  - Amygdala/metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Corticosterone/blood
MH  - Frontal Lobe/metabolism
MH  - Hippocampus/metabolism
MH  - Hypothalamo-Hypophyseal System/*metabolism
MH  - Hypothalamus/metabolism
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Pituitary Gland/metabolism
MH  - Pituitary-Adrenal System/*metabolism
MH  - RNA, Small Interfering/administration & dosage/pharmacology
MH  - Rats
MH  - Restraint, Physical
OTO - NOTNLM
OT  - ACTH
OT  - BDNF knockdown mice
OT  - corticosterone
OT  - neurotrophin
OT  - siRNA rat
EDAT- 2015/09/22 06:00
MHDA- 2016/08/19 06:00
CRDT- 2015/09/22 06:00
PHST- 2015/01/09 00:00 [received]
PHST- 2015/09/03 00:00 [revised]
PHST- 2015/09/13 00:00 [accepted]
PHST- 2015/09/22 06:00 [entrez]
PHST- 2015/09/22 06:00 [pubmed]
PHST- 2016/08/19 06:00 [medline]
AID - 10.1111/jne.12324 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2015 Nov;27(11):850-60. doi: 10.1111/jne.12324.