PMID- 26390821
OWN - NLM
STAT- MEDLINE
DCOM- 20160526
LR  - 20181202
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 180
IP  - 3-4
DP  - 2015 Nov 18
TI  - The pathogenic role of torque teno sus virus 1 and 2 and their correlations with 
      various viral pathogens and host immunocytes in wasting pigs.
PG  - 186-95
LID - S0378-1135(15)30017-1 [pii]
LID - 10.1016/j.vetmic.2015.08.027 [doi]
AB  - The pathogenic role of torque teno sus virus (TTSuV) in swine is controversial 
      among different studies. The present study intended to evaluate the potential 
      pathogenicity of TTSuV based on its correlations with the histopathological 
      changes, various common concurrently infected viral pathogens including porcine 
      circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus 
      (PRRSV), and porcine parvovirus (PPV), as well as changes in the distribution and 
      population of host immunocytes such as B lymphocytes, T lymphocytes, and 
      macrophages by using the superficial inguinal lymph nodes (siLNs) of wasting 
      pigs. A tissue microarray consisting of 270 available siLNs collected from 262 
      clinically wasting and 8 healthy pigs, respectively, were used for the detection 
      of TTSuV1, TTSuV2, PCV2, PRRSV, and PPV by either in situ hybridization (ISH) or 
      immunohistochemical (IHC) staining, and for the detection of various subsets of 
      immunocytes by IHC staining with monoclonal antibodies to CD3, CD79a, and 
      lysozyme. The slides were then subject to digital scanning followed by a 
      semi-quantitative positive pixel evaluation for further statistical analysis. 
      Although a high prevalence of TTSuV1 and/or TTSuV2 infection was noted in both 
      wasting and healthy pigs, the wasting pigs had a significantly higher intensity 
      in both TTSuV1 and TTSuV2 ISH-positive signals than healthy ones did. In the 
      wasting pigs, a significant positive correlation in the tissue viral load was 
      noted between TTSuV1 and TTSuV2 and between TTSuV2 and PCV2, but not between 
      TTSuV1 and PCV2. Conversely, a significant negative correlation in the tissue 
      viral load was revealed between TTSuV2, but not TTSuV1, and PRRSV. The tissue 
      viral load of TTSuV1 was significantly correlated with B cell hyperplasia, while 
      the tissue viral load of TTSuV2 was significantly correlated with increased 
      macrophage population. The ISH positivity of TTSuV2 was significantly correlated 
      with lymphoid depletion and granulomatous inflammation, which are the 
      characteristic histopathological findings in postweaning multisystemic wasting 
      syndrome-affected pigs. These findings suggest that both TTSuV species may have 
      the potential involving the development of porcine circovirus-associated lymphoid 
      lesions via alternating the host immune system.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Lee, Yao
AU  - Lee Y
AD  - Graduate Institute of Molecular and Comparative Pathobiology, School of 
      Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, ROC; 
      Department of Pathobiology and Diagnostic Investigation, College of Veterinary 
      Medicine, Michigan State University, East Lansing, MI 48824, USA.
FAU - Lin, Chun-Ming
AU  - Lin CM
AD  - Graduate Institute of Molecular and Comparative Pathobiology, School of 
      Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, ROC.
FAU - Jeng, Chian-Ren
AU  - Jeng CR
AD  - Graduate Institute of Molecular and Comparative Pathobiology, School of 
      Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, ROC.
FAU - Chang, Hui-Wen
AU  - Chang HW
AD  - Graduate Institute of Molecular and Comparative Pathobiology, School of 
      Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, ROC.
FAU - Chang, Chih-Cheng
AU  - Chang CC
AD  - Department of Veterinary Medicine, National Chiayi University, Chiayi 60054, 
      Taiwan, ROC. Electronic address: ccchang@mail.ncyu.edu.tw.
FAU - Pang, Victor Fei
AU  - Pang VF
AD  - Graduate Institute of Molecular and Comparative Pathobiology, School of 
      Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan, ROC. 
      Electronic address: pang@ntu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150829
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/immunology
MH  - Circoviridae Infections/virology
MH  - Circovirus/pathogenicity
MH  - Coinfection/virology
MH  - DNA Virus Infections/immunology/*veterinary/virology
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Inflammation/immunology/virology
MH  - Lymph Nodes/pathology
MH  - Macrophages/immunology
MH  - Male
MH  - Parvovirus, Porcine/pathogenicity
MH  - Porcine respiratory and reproductive syndrome virus/pathogenicity
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - T-Lymphocytes/immunology
MH  - Tissue Array Analysis
MH  - Torque teno virus/*pathogenicity
MH  - Viral Load
MH  - Wasting Syndrome/immunology/*veterinary/virology
OTO - NOTNLM
OT  - In situ hybridization (ISH)
OT  - Porcine circovirus type 2 (PCV2)
OT  - Superficial inguinal lymph node (siLN)
OT  - Torque teno sus virus (TTSuV)
OT  - Wasting pig
EDAT- 2015/09/24 06:00
MHDA- 2016/05/27 06:00
CRDT- 2015/09/23 06:00
PHST- 2014/06/19 00:00 [received]
PHST- 2015/08/26 00:00 [revised]
PHST- 2015/08/27 00:00 [accepted]
PHST- 2015/09/23 06:00 [entrez]
PHST- 2015/09/24 06:00 [pubmed]
PHST- 2016/05/27 06:00 [medline]
AID - S0378-1135(15)30017-1 [pii]
AID - 10.1016/j.vetmic.2015.08.027 [doi]
PST - ppublish
SO  - Vet Microbiol. 2015 Nov 18;180(3-4):186-95. doi: 10.1016/j.vetmic.2015.08.027. 
      Epub 2015 Aug 29.