PMID- 26394808
OWN - NLM
STAT- MEDLINE
DCOM- 20160722
LR  - 20220321
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 54
IP  - 12
DP  - 2015 Dec
TI  - New data shed light on Y-loss-related pathogenesis in myelodysplastic syndromes.
PG  - 717-24
LID - 10.1002/gcc.22282 [doi]
AB  - Loss of the Y-chromosome (LOY) is described as both a normal age-related event 
      and a marker of a neoplastic clone in hematologic diseases. To assess the 
      significance of LOY in myelodysplastic syndromes (MDS), we determined the 
      percentage of LOY in clonal CD34+ peripheral blood cells in comparison to normal 
      CD3+ T-cells of 27 MDS patients using fluorescence in situ hybridization (FISH) 
      analysis. Results were compared with the percentage of LOY in CD34+ and CD3+ 
      cells of 32 elderly men without hematologic diseases and in 25 young blood 
      donors. While LOY could not be detected in CD3+ cells of young men, it was 
      observed in CD3+ cells of elderly men without hematologic diseases (2.5% LOY) as 
      well as in CD3+ cells of elderly MDS patients (5.8% LOY). The percentage of CD34+ 
      cells affected by LOY was significantly higher in MDS patients compared to 
      elderly men without hematologic diseases (43.3% vs. 13.2%, P = 0.005), indicating 
      that LOY has an age-related basis but is also associated with MDS. Furthermore, 
      we aimed to define a threshold between age- and disease-associated LOY in MDS. 
      Statistical analysis revealed that a value of 21.5% LOY in CD34+ peripheral blood 
      cells provided the best threshold to discriminate between these two conditions in 
      MDS. We conclude that LOY is clonal in a substantial number of MDS based on an 
      age-related predisposition.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Ganster, Christina
AU  - Ganster C
AD  - Department of Hematology and Medical Oncology, University Medical Center 
      Gottingen, Gottingen, Germany.
FAU - Kampfe, Dietrich
AU  - Kampfe D
AD  - Praxis fur Hamatologie und Onkologie, Ludenscheid, Germany.
FAU - Jung, Klaus
AU  - Jung K
AD  - Department of Medical Statistics, University Medical Center Gottingen, Gottingen, 
      Germany.
FAU - Braulke, Friederike
AU  - Braulke F
AD  - Department of Hematology and Medical Oncology, University Medical Center 
      Gottingen, Gottingen, Germany.
FAU - Shirneshan, Katayoon
AU  - Shirneshan K
AD  - Department of Hematology and Medical Oncology, University Medical Center 
      Gottingen, Gottingen, Germany.
FAU - Machherndl-Spandl, Sigrid
AU  - Machherndl-Spandl S
AD  - 1st Medical Department with Hematology, Stem Cell Transplantation, Hemostasis and 
      Medical Oncology, Elisabethinen Hospital, Linz, Austria.
FAU - Suessner, Susanne
AU  - Suessner S
AD  - Red Cross Blood Transfusion Service of Upper Austria, Austrian Cluster for Tissue 
      Regeneration, Linz, Austria.
FAU - Bramlage, Carsten P
AU  - Bramlage CP
AD  - Department of Nephrology and Rheumatology, University Medical Center Gottingen, 
      Gottingen, Germany.
FAU - Legler, Tobias J
AU  - Legler TJ
AD  - Department of Transfusion Medicine, University Medical Center Gottingen, 
      Gottingen, Germany.
FAU - Koziolek, Michael J
AU  - Koziolek MJ
AD  - Department of Nephrology and Rheumatology, University Medical Center Gottingen, 
      Gottingen, Germany.
FAU - Haase, Detlef
AU  - Haase D
AD  - Department of Hematology and Medical Oncology, University Medical Center 
      Gottingen, Gottingen, Germany.
FAU - Schanz, Julie
AU  - Schanz J
AD  - Department of Hematology and Medical Oncology, University Medical Center 
      Gottingen, Gottingen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150923
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Antigens, CD34)
RN  - 0 (CD3 Complex)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD34/metabolism
MH  - Blood Donors
MH  - CD3 Complex/metabolism
MH  - Cells, Cultured
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Y/*genetics
MH  - Clonal Selection, Antigen-Mediated
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - T-Lymphocytes/immunology/metabolism
MH  - Young Adult
EDAT- 2015/09/24 06:00
MHDA- 2016/07/23 06:00
CRDT- 2015/09/24 06:00
PHST- 2015/03/05 00:00 [received]
PHST- 2015/06/18 00:00 [revised]
PHST- 2015/06/18 00:00 [accepted]
PHST- 2015/09/24 06:00 [entrez]
PHST- 2015/09/24 06:00 [pubmed]
PHST- 2016/07/23 06:00 [medline]
AID - 10.1002/gcc.22282 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2015 Dec;54(12):717-24. doi: 10.1002/gcc.22282. Epub 
      2015 Sep 23.