PMID- 26395262 OWN - NLM STAT- MEDLINE DCOM- 20170130 LR - 20190221 IS - 1423-0380 (Electronic) IS - 1010-4283 (Linking) VI - 37 IP - 2 DP - 2016 Feb TI - Tumor necrosis factor superfamily member 13 is a novel biomarker for diagnosis and prognosis and promotes cancer cell proliferation in laryngeal squamous cell carcinoma. PG - 2635-45 LID - 10.1007/s13277-015-4016-8 [doi] AB - Tumor necrosis factor superfamily member 13 (TNFSF13) modulates cell proliferation and apoptosis and participates in the pathogenesis of solid tumors, but its role in laryngeal cancer development is not clearly defined. In order to investigate whether TNFSF13 can be used as a biomarker for diagnosis and prognosis in laryngeal squamous cell carcinoma (LSCC) and the role of TNFSF13 in laryngeal cancer carcinogenesis, we conducted immunohistochemistry and ELISA assays to evaluate the expression level of TNFSF13 in laryngeal cancer patients and the contrast. We also conducted experiments on the functional study of TNFSF13 in vitro. We found that the expression levels of TNFSF13, ki-67, and NF-kappaB p65 in LSCC tumor tissues were higher than those in vocal polyp and para-carcinoma tissues. The Spearman rank correlation analysis showed that the expression of TNFSF13 had a positive correlation with the expression of ki-67 and NF-kappaB p65. Cox regression analysis and Kaplan-Meier plots confirmed the expression level of TNFSF13 was a prognostic factor for LSCC. Moreover, the serum TNFSF13 level was significantly higher in LSCC patients than in the controls, and the serum expression level of TNFSF13 can distinguish LSCC from healthy people, precancerosis, or laryngeal benign tumor. In addition, functional study of TNFSF13 in vitro revealed that knockdown of TNFSF13 inhibited cell proliferation by inducing G1 phase cell cycle arrest in Hep-2 cells. In conclusion, TNFSF13 may be a potential novel molecular target for diagnosis and prognosis in human LSCC, and therapies that target TNFSF13 may have clinical significance for the treatment of LSCC. FAU - Wang, Ru AU - Wang R AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. FAU - Guo, Yichao AU - Guo Y AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. FAU - Ma, Hongzhi AU - Ma H AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing, 100005, China. FAU - Feng, Lin AU - Feng L AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing, 100005, China. FAU - Wang, Qi AU - Wang Q AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing, 100005, China. FAU - Chen, Xiaohong AU - Chen X AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing, 100005, China. FAU - Lian, Meng AU - Lian M AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. FAU - Wang, Haizhou AU - Wang H AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. FAU - Fang, Jugao AU - Fang J AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China. fangjg19651110@163.com. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing, 100005, China. fangjg19651110@163.com. AD - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 100730, China. fangjg19651110@163.com. LA - eng PT - Journal Article DEP - 20150923 PL - Netherlands TA - Tumour Biol JT - Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine JID - 8409922 RN - 0 (Biomarkers, Tumor) RN - 0 (NF-kappa B) RN - 0 (TNFSF13 protein, human) RN - 0 (Tumor Necrosis Factor Ligand Superfamily Member 13) SB - IM MH - Aged MH - Apoptosis/genetics MH - Biomarkers, Tumor/*genetics MH - Carcinoma, Squamous Cell/*genetics/*pathology MH - Cell Line MH - Cell Line, Tumor MH - Cell Proliferation/*genetics MH - Female MH - G1 Phase/genetics MH - Gene Expression Regulation, Neoplastic MH - HEK293 Cells MH - Head and Neck Neoplasms/genetics/pathology MH - Humans MH - Immunohistochemistry/methods MH - Laryngeal Neoplasms/*genetics/*pathology MH - Male MH - NF-kappa B/genetics MH - Prognosis MH - Squamous Cell Carcinoma of Head and Neck MH - Tumor Necrosis Factor Ligand Superfamily Member 13/*genetics OTO - NOTNLM OT - Biomarker OT - Laryngeal neoplasms OT - NF-kappaB p65 OT - TNFSF13 OT - ki-67 EDAT- 2015/09/24 06:00 MHDA- 2017/01/31 06:00 CRDT- 2015/09/24 06:00 PHST- 2015/06/26 00:00 [received] PHST- 2015/08/28 00:00 [accepted] PHST- 2015/09/24 06:00 [entrez] PHST- 2015/09/24 06:00 [pubmed] PHST- 2017/01/31 06:00 [medline] AID - 10.1007/s13277-015-4016-8 [pii] AID - 10.1007/s13277-015-4016-8 [doi] PST - ppublish SO - Tumour Biol. 2016 Feb;37(2):2635-45. doi: 10.1007/s13277-015-4016-8. Epub 2015 Sep 23.