PMID- 26396995
OWN - NLM
STAT- MEDLINE
DCOM- 20160512
LR  - 20161125
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 140
IP  - 21
DP  - 2015 Nov 7
TI  - A FRET-based ratiometric fluorescent aptasensor for rapid and onsite visual 
      detection of ochratoxin A.
PG  - 7434-42
LID - 10.1039/c5an01403d [doi]
AB  - A color change observable by the naked eye to indicate the content of an analyte 
      is considered to be the most conceivable way of various sensing protocols. By 
      taking advantage of the Forster resonance energy transfer (FRET) principles, we 
      herein designed a dual-emission ratiometric fluorescent aptasensor for ochratoxin 
      A (OTA) detection via a dual mode of fluorescent sensing and onsite visual 
      screening. Amino group-modified OTA's aptamer was firstly labeled with the 
      green-emitting CdTe quantum dots (gQDs) donor. The red-emitting CdTe QDs (rQDs) 
      which were wrapped in the silica sphere could serve as the reference signal, 
      while the gold nanoparticle (AuNP) acceptors were attached on the silica surface 
      to bind with the thiolated complementary DNA (cDNA). The hybridization reaction 
      between the aptamer and the cDNA brought gQD-AuNP pair close enough, thereby 
      making the FRET occur in the aptasensor fabrication, while the subsequent 
      fluorescence recovery induced by OTA was obtained in the detection procedure. 
      Based on the red background of the wrapped rQDs, the aptasensor in response to 
      increasing OTA displayed a distinguishable color change from red to yellow-green, 
      which could be conveniently readout in solution even by the naked eye. Since the 
      bioconjugations used as the aptasensor can be produced at large scale, this 
      method can be used for in situ, rapid, or high-throughput OTA detection after 
      only an incubation step in a homogeneous mode. We believe that this novel 
      aptasensing strategy provides not only a promising method for OTA detection but 
      also a universal model for detecting diverse targets by changing the 
      corresponding aptamer.
FAU - Qian, Jing
AU  - Qian J
AD  - Key Laboratory of Modern Agriculture Equipment and Technology, School of 
      Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P. R. 
      China. wangkun@ujs.edu.cn.
FAU - Wang, Kan
AU  - Wang K
FAU - Wang, Chengquan
AU  - Wang C
FAU - Hua, Mengjuan
AU  - Hua M
FAU - Yang, Zhenting
AU  - Yang Z
FAU - Liu, Qian
AU  - Liu Q
FAU - Mao, Hanping
AU  - Mao H
FAU - Wang, Kun
AU  - Wang K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Cadmium Compounds)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Ochratoxins)
RN  - 1779SX6LUY (ochratoxin A)
RN  - 7440-57-5 (Gold)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - NQA0O090ZJ (Tellurium)
RN  - STG188WO13 (cadmium telluride)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Cadmium Compounds/chemistry
MH  - *Color
MH  - Colorimetry/*instrumentation/methods
MH  - DNA, Complementary/metabolism
MH  - Fluorescence Resonance Energy Transfer/methods
MH  - Fluorescent Dyes/*chemistry
MH  - Food Analysis/*methods
MH  - Food Contamination
MH  - Gold/chemistry
MH  - Metal Nanoparticles/chemistry
MH  - Microscopy, Electron, Transmission
MH  - Nucleic Acid Hybridization
MH  - Ochratoxins/*analysis
MH  - Quantum Dots
MH  - Reproducibility of Results
MH  - Silicon Dioxide/chemistry
MH  - Spectrometry, Fluorescence
MH  - Tellurium/chemistry
MH  - Wine/analysis
EDAT- 2015/09/24 06:00
MHDA- 2016/05/14 06:00
CRDT- 2015/09/24 06:00
PHST- 2015/09/24 06:00 [entrez]
PHST- 2015/09/24 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
AID - 10.1039/c5an01403d [doi]
PST - ppublish
SO  - Analyst. 2015 Nov 7;140(21):7434-42. doi: 10.1039/c5an01403d.