PMID- 26398771
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20230609
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 34
IP  - 6
DP  - 2015 Dec
TI  - Downregulation of Notch1 induces apoptosis and inhibits cell proliferation and 
      metastasis in laryngeal squamous cell carcinoma.
PG  - 3111-9
LID - 10.3892/or.2015.4274 [doi]
AB  - Notch signaling plays a key role in a wide variety of human tumors; it can be an 
      oncogene or a tumor-suppressor gene depending on the tissue context. The 
      functions of Notch1 in laryngeal squamous cell carcinoma (LSCC) are largely 
      unknown. We investigated the role of Notch1 in LSCC cell growth, apoptosis and 
      metastasis. We analyzed Notch1 expression in clinical LSCC samples using quantum 
      dot immunohistochemistry (QD-IHC) and conventional IHC. Human laryngeal carcinoma 
      HEp-2 cells were transfected with Notch1-specific short hairpin RNA (shRNA), and 
      cell proliferation, apoptosis, and migration and invasion were evaluated using 
      the cell counting assay, flow cytometry and wound healing and Transwell assays, 
      respectively; western blotting was used to validate the expression of Notch1 
      target genes. Compared with normal tissues, Notch1 was upregulated in LSCC 
      tissues; compared with LSCC tissues without metastasis, Notch1 upregulation was 
      enhanced in LSCC tissues with metastasis (P<0.05). Transfection downregulated 
      Notch1 mRNA and protein expression levels in the Notch1 shRNA group. There was a 
      significantly greater decrease in cell proliferation in the Notch1 shRNA group 
      than cell proliferation in the non-transfected (P<0.05) and negative shRNA groups 
      (P<0.05). Furthermore, Notch1 knockdown induced apoptosis in the HEp-2 cells. 
      Additionally, the number of migrated and invasive cells in the Notch1 shRNA group 
      was decreased (P<0.05). Notch1 knockdown in the HEp-2 cells greatly inhibited 
      phosphorylated extracellular signal-related kinase (p-ERK), phosphorylated 
      protein kinase B (p-AKT), c-Myc, Bcl-2, p21, cyclin D1, cyclin-dependent kinase 4 
      (CDK4) and cyclin E expression levels and increased Bax expression. Altogether, 
      our findings indicate that Notch1 expression is increased in human LSCC and 
      correlates with tumorigenesis and metastasis, while in HEp-2 cells, Notch1 
      knockdown inhibited cell growth, induced apoptosis and inhibited migration and 
      invasion by regulating Notch1 target genes, suggesting it may be a potential 
      therapeutic target for treating LSCC.
FAU - Dai, Meng-Yuan
AU  - Dai MY
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
FAU - Fang, Fang
AU  - Fang F
AD  - Department of Medical Market, Renmin Hospital of Wuhan University, Wuhan 430060, 
      P.R. China.
FAU - Zou, You
AU  - Zou Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
FAU - Yi, Xing
AU  - Yi X
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
FAU - Ding, Yong-Jun
AU  - Ding YJ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
FAU - Tao, Ze-Zhang
AU  - Tao ZZ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
FAU - Chen, Shi-Ming
AU  - Chen SM
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan 430060, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150915
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (NOTCH1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Receptor, Notch1)
SB  - IM
EIN - Oncol Rep. 2023 Jul;50(1):. PMID: 37293860
MH  - Apoptosis/genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Neoplasm Invasiveness/genetics
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/*biosynthesis
MH  - Receptor, Notch1/*biosynthesis/genetics
MH  - Signal Transduction/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
EDAT- 2015/09/24 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/09/24 06:00
PHST- 2015/06/29 00:00 [received]
PHST- 2015/07/22 00:00 [accepted]
PHST- 2015/09/24 06:00 [entrez]
PHST- 2015/09/24 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.3892/or.2015.4274 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 Dec;34(6):3111-9. doi: 10.3892/or.2015.4274. Epub 2015 Sep 15.