PMID- 26398903
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20220321
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 34
IP  - 12
DP  - 2015 Dec
TI  - The Endoribonuclease Activity Essential for the Nonstructural Protein 11 of 
      Porcine Reproductive and Respiratory Syndrome Virus to Inhibit NLRP3 
      Inflammasome-Mediated IL-1beta Induction.
PG  - 728-35
LID - 10.1089/dna.2015.2929 [doi]
AB  - NLRP3 inflammasome, which is multiprotein complex that induces the maturity and 
      secretion of proinflammatory interleukin-1beta (IL-1beta), takes a bridge between the 
      innate and adaptive immune responses to the invading pathogens. It has been shown 
      that porcine reproductive and respiratory syndrome virus (PRRSV) could activate 
      the NLRP3 inflammasome but induce the host's immunosuppression. This study aims 
      to explore whether PRRSV could encode the component to antagonize the NLRP3 
      inflammasome. The obtained results showed that PRRSV could induce the expression 
      and secretion of IL-1beta in early infection through the pathway of NLRP3 
      inflammasome in porcine alveolar macrophages (PAMs), but the levels of pro-IL-1beta 
      mRNA and IL-1beta protein decreased to a degree that was similar to the level of the 
      mock-infected group in later infection. This work also found that PRRSV 
      nonstructural protein (nsp) 11 could inhibit the expression of pro-IL-1beta mRNA 
      induced by lipopolysaccharide (LPS) and the secretion of IL-1beta induced by LPS 
      plus nigericin in PAMs. Furthermore, the mutation studies showed that the 
      endoribonuclease activity was essential for nsp11 to inhibit the secretion of 
      IL-1beta. Therefore, it could be indicated that PRRSV could induce the activation of 
      NLRP3 inflammasome, but the virus encoded nsp11 to inhibit this action.
FAU - Wang, Chao
AU  - Wang C
AD  - 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .
AD  - 2 Henan Provincial Key Laboratory of Animal Immunology , Henan Academy of 
      Agricultural Sciences, Zhengzhou, China .
FAU - Shi, Xibao
AU  - Shi X
AD  - 2 Henan Provincial Key Laboratory of Animal Immunology , Henan Academy of 
      Agricultural Sciences, Zhengzhou, China .
AD  - 3 College of Life Sciences, Henan Normal University , Xinxiang, China .
FAU - Zhang, Xiaozhuan
AU  - Zhang X
AD  - 3 College of Life Sciences, Henan Normal University , Xinxiang, China .
FAU - Wang, Aiping
AU  - Wang A
AD  - 4 Department of Bioengineering, Zhengzhou University , Zhengzhou, China .
FAU - Wang, Li
AU  - Wang L
AD  - 2 Henan Provincial Key Laboratory of Animal Immunology , Henan Academy of 
      Agricultural Sciences, Zhengzhou, China .
FAU - Chen, Jing
AU  - Chen J
AD  - 2 Henan Provincial Key Laboratory of Animal Immunology , Henan Academy of 
      Agricultural Sciences, Zhengzhou, China .
FAU - Deng, Ruiguang
AU  - Deng R
AD  - 2 Henan Provincial Key Laboratory of Animal Immunology , Henan Academy of 
      Agricultural Sciences, Zhengzhou, China .
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - 1 College of Veterinary Medicine, Northwest A&F University , Yangling, China .
AD  - 2 Henan Provincial Key Laboratory of Animal Immunology , Henan Academy of 
      Agricultural Sciences, Zhengzhou, China .
AD  - 5 College of Animal Science and Veterinary Medicine, Henan Agricultural 
      University , Zhengzhou, China .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150923
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Carrier Proteins)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLRP3 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.1.- (Endoribonucleases)
SB  - IM
MH  - Animals
MH  - Arterivirus/metabolism/*physiology
MH  - Carrier Proteins/*metabolism
MH  - Endoribonucleases/*metabolism
MH  - Inflammasomes/*metabolism
MH  - Interleukin-1beta/*biosynthesis/genetics
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - RNA, Messenger/genetics
MH  - Swine
MH  - Viral Nonstructural Proteins/*metabolism
EDAT- 2015/09/24 06:00
MHDA- 2016/04/20 06:00
CRDT- 2015/09/24 06:00
PHST- 2015/09/24 06:00 [entrez]
PHST- 2015/09/24 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
AID - 10.1089/dna.2015.2929 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2015 Dec;34(12):728-35. doi: 10.1089/dna.2015.2929. Epub 2015 Sep 
      23.