PMID- 26399683
OWN - NLM
STAT- MEDLINE
DCOM- 20160408
LR  - 20151016
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 467
IP  - 1
DP  - 2015 Nov 6
TI  - Bropirimine inhibits osteoclast differentiation through production of 
      interferon-beta.
PG  - 146-51
LID - S0006-291X(15)30609-4 [pii]
LID - 10.1016/j.bbrc.2015.09.092 [doi]
AB  - Bropirimine is a synthetic agonist for toll-like receptor 7 (TLR7). In this 
      study, we investigated the effects of bropirimine on differentiation and 
      bone-resorbing activity of osteoclasts in vitro. Bropirimine inhibited osteoclast 
      differentiation of mouse bone marrow-derived macrophages (BMMs) induced by 
      receptor activator of nuclear factor kappaB ligand (RANKL) in a 
      concentration-dependent manner. Furthermore, it suppressed the mRNA expression of 
      nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 
      (NFATc1), a master transcription factor for osteoclast differentiation, without 
      affecting BMM viability. Bropirimine also inhibited osteoclast differentiation 
      induced in co-cultures of mouse bone marrow cells (BMCs) and mouse osteoblastic 
      UAMS-32 cells in the presence of activated vitamin D3. Bropirimine partially 
      suppressed the expression of RANKL mRNA in UAMS-32 cells induced by activated 
      vitamin D3. Finally, the anti-interferon-beta (IFN-beta) antibody restored 
      RANKL-dependent differentiation of BMMs into osteoclasts suppressed by 
      bropirimine. These results suggest that bropirimine inhibits differentiation of 
      osteoclast precursor cells into osteoclasts via TLR7-mediated production of 
      IFN-beta.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Suzuki, Hiroaki
AU  - Suzuki H
AD  - Department of Biochemistry, Showa University School of Dentistry, Tokyo 142-8555, 
      Japan.
FAU - Mochizuki, Ayako
AU  - Mochizuki A
AD  - Department of Oral Physiology, Showa University School of Dentistry, Tokyo 
      142-8555, Japan.
FAU - Yoshimura, Kentaro
AU  - Yoshimura K
AD  - Department of Biochemistry, Showa University School of Dentistry, Tokyo 142-8555, 
      Japan.
FAU - Miyamoto, Yoichi
AU  - Miyamoto Y
AD  - Department of Biochemistry, Showa University School of Dentistry, Tokyo 142-8555, 
      Japan.
FAU - Kaneko, Kotaro
AU  - Kaneko K
AD  - Department of Biochemistry, Showa University School of Dentistry, Tokyo 142-8555, 
      Japan; Department of Oral and Maxillofacial Surgery, Tokyo Medical University, 
      Tokyo 160-0023, Japan.
FAU - Inoue, Tomio
AU  - Inoue T
AD  - Department of Oral Physiology, Showa University School of Dentistry, Tokyo 
      142-8555, Japan.
FAU - Chikazu, Daichi
AU  - Chikazu D
AD  - Department of Oral and Maxillofacial Surgery, Tokyo Medical University, Tokyo 
      160-0023, Japan.
FAU - Takami, Masamichi
AU  - Takami M
AD  - Department of Pharmacology, Showa University School of Dentistry, Tokyo 142-8555, 
      Japan.
FAU - Kamijo, Ryutaro
AU  - Kamijo R
AD  - Department of Biochemistry, Showa University School of Dentistry, Tokyo 142-8555, 
      Japan. Electronic address: kamijor@dent.showa-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150921
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr7 protein, mouse)
RN  - 0 (Tnfsf11 protein, mouse)
RN  - 0 (Toll-Like Receptor 7)
RN  - 77238-31-4 (Interferon-beta)
RN  - 8J337D1HZY (Cytosine)
RN  - J57CTF25XJ (bropirimine)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Line
MH  - Coculture Techniques
MH  - Cytosine/*analogs & derivatives/pharmacology
MH  - Interferon-beta/*biosynthesis
MH  - Macrophages/cytology/drug effects/immunology
MH  - Male
MH  - Membrane Glycoproteins/agonists
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoclasts/*cytology/*drug effects/immunology
MH  - RANK Ligand/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Toll-Like Receptor 7/agonists
OTO - NOTNLM
OT  - Differentiation
OT  - Interferon-beta
OT  - Osteoclasts
OT  - Toll-like receptor-7
EDAT- 2015/09/25 06:00
MHDA- 2016/04/09 06:00
CRDT- 2015/09/25 06:00
PHST- 2015/09/02 00:00 [received]
PHST- 2015/09/17 00:00 [accepted]
PHST- 2015/09/25 06:00 [entrez]
PHST- 2015/09/25 06:00 [pubmed]
PHST- 2016/04/09 06:00 [medline]
AID - S0006-291X(15)30609-4 [pii]
AID - 10.1016/j.bbrc.2015.09.092 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Nov 6;467(1):146-51. doi: 
      10.1016/j.bbrc.2015.09.092. Epub 2015 Sep 21.