PMID- 26400933
OWN - NLM
STAT- MEDLINE
DCOM- 20151229
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 35
IP  - 38
DP  - 2015 Sep 23
TI  - Dendritic Organization of Olfactory Inputs to Medial Amygdala Neurons.
PG  - 13020-8
LID - 10.1523/JNEUROSCI.0627-15.2015 [doi]
AB  - The medial amygdala (MeA) is a central hub in the olfactory neural network. It 
      receives vomeronasal information directly from the accessory olfactory bulb (AOB) 
      and main olfactory information largely via odor-processing regions such as the 
      olfactory cortical amygdala (CoA). How these inputs are processed by MeA neurons 
      is poorly understood. Using the GAD67-GFP mouse, we show that MeA principal 
      neurons receive convergent AOB and CoA inputs. Somatically recorded AOB synaptic 
      inputs had slower kinetics than CoA inputs, suggesting that they are 
      electrotonically more distant. Field potential recording, pharmacological 
      manipulation, and Ca(2+) imaging revealed that AOB synapses are confined to 
      distal dendrites and segregated from the proximally located CoA synapses. 
      Moreover, unsynchronized AOB inputs had significantly broader temporal summation 
      that was dependent on the activation of NMDA receptors. These findings show that 
      MeA principal neurons process main and accessory olfactory inputs differentially 
      in distinct dendritic compartments. Significance statement: In most vertebrates, 
      olfactory cues are processed by two largely segregated neural pathways, the main 
      and accessory olfactory systems, which are specialized to detect odors and 
      nonvolatile chemosignals, respectively. Information from these two pathways 
      ultimately converges at higher brain regions, one of the major hubs being the 
      medial amygdala. Little is known about how olfactory inputs are processed by 
      medial amygdala neurons. This study shows that individual principal neurons in 
      this region receive input from both pathways and that these synapses are 
      spatially segregated on their dendritic tree. We provide evidence suggesting that 
      this dendritic segregation leads to distinct input integration and impact on 
      neuronal output; hence, dendritic mechanisms control olfactory processing in the 
      amygdala.
CI  - Copyright (c) 2015 the authors 0270-6474/15/3513020-09$15.00/0.
FAU - Keshavarzi, Sepideh
AU  - Keshavarzi S
AD  - Queensland Brain Institute, The University of Queensland, St. Lucia, Queensland 
      4072, Australia.
FAU - Power, John M
AU  - Power JM
AD  - Queensland Brain Institute, The University of Queensland, St. Lucia, Queensland 
      4072, Australia.
FAU - Albers, Eva H H
AU  - Albers EH
AD  - Queensland Brain Institute, The University of Queensland, St. Lucia, Queensland 
      4072, Australia.
FAU - Sullivan, Robert K S
AU  - Sullivan RK
AD  - Queensland Brain Institute, The University of Queensland, St. Lucia, Queensland 
      4072, Australia.
FAU - Sah, Pankaj
AU  - Sah P
AD  - Queensland Brain Institute, The University of Queensland, St. Lucia, Queensland 
      4072, Australia pankaj.sah@uq.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Quinoxalines)
RN  - 0 (Sodium Channel Blockers)
RN  - 118876-58-7 (2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline)
RN  - 124-87-8 (Picrotoxin)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 76326-31-3 (2-amino-5-phosphopentanoic acid)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 1)
RN  - HG18B9YRS7 (Valine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - J Neurosci. 2016 Feb 17;36(7):2083-5. PMID: 26888920
MH  - Action Potentials/drug effects/genetics
MH  - Afferent Pathways/drug effects/*physiology
MH  - Animals
MH  - Calcium/metabolism
MH  - Central Nervous System Stimulants/pharmacology
MH  - Corticomedial Nuclear Complex/*cytology
MH  - Dendrites/drug effects/*physiology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects/genetics
MH  - Glutamate Decarboxylase/genetics/metabolism
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, Transgenic
MH  - Neurons/*cytology/physiology
MH  - Olfactory Bulb/*physiology
MH  - Patch-Clamp Techniques
MH  - Picrotoxin/pharmacology
MH  - Quinoxalines/pharmacology
MH  - Sodium Channel Blockers/pharmacology
MH  - Tetrodotoxin/pharmacology
MH  - Valine/analogs & derivatives/pharmacology
PMC - PMC6605442
OTO - NOTNLM
OT  - dendrite
OT  - emotion
OT  - mating
OT  - olfaction
OT  - reproduction
EDAT- 2015/09/25 06:00
MHDA- 2015/12/30 06:00
PMCR- 2016/03/23
CRDT- 2015/09/25 06:00
PHST- 2015/09/25 06:00 [entrez]
PHST- 2015/09/25 06:00 [pubmed]
PHST- 2015/12/30 06:00 [medline]
PHST- 2016/03/23 00:00 [pmc-release]
AID - 35/38/13020 [pii]
AID - 0627-15 [pii]
AID - 10.1523/JNEUROSCI.0627-15.2015 [doi]
PST - ppublish
SO  - J Neurosci. 2015 Sep 23;35(38):13020-8. doi: 10.1523/JNEUROSCI.0627-15.2015.