PMID- 26400954
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20170103
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 54
IP  - 3
DP  - 2016 Mar
TI  - Simultaneous Determination of 10 Flavonoids in Crude and Wine-Processed Radix 
      scutellariae by UHPLC.
PG  - 312-7
LID - 10.1093/chromsci/bmv143 [doi]
AB  - Radix scutellariae is a well-known traditional Chinese medicine used for the 
      treatments of inflammation, pyrexia, hepatitis, etc. Flavonoids are its main 
      active compounds. The aim of this study is to develop and validate the ultra 
      high-performance liquid chromatography (UHPLC) method for simultaneous 
      determination of 10 flavonoids (baicalin, wogonoside, baicalein, wogonin, 
      oroxylin A, chrysin, scutellarin, oroxylin A-7-O-glucuronide, apigenin and 
      apigenin-7-glucuronide) in crude and wine-processed R. scutellariae. The 
      quantitative determination was conducted by UHPLC. Optimal separation was 
      achieved by gradient elution with mobile phase consisting of 0.01% aqueous formic 
      acid and methanol on a Waters ACQUITY UHPLC BEH C18 column. Detection wavelength 
      was set at 275 nm. Method validation was accomplished with linearity, precision 
      and recovery tests. All calibration curves showed good linearity (R(2) > 0.9993). 
      The limit of detection and limit of quantification of these compounds were from 
      0.08 to 0.24 microg/mL and from 0.23 to 0.76 microg/mL, respectively. The average 
      recoveries of these compounds were from 96.95 to 109.51% with relative standard 
      deviation (RSD) values from 2.14 to 3.26% for crude R. scutellariae, while from 
      94.73 to 108.38% with RSD values from 1.83 to 3.47% for wine-processed R. 
      scutellariae. The developed method can be applied to the intrinsic quality 
      control of crude and wine-processed R. scutellariae.
CI  - (c) The Author 2015. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Cui, Xiaobing
AU  - Cui X
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China Jiangsu Provincial TCM Engineering Technology Research Center of High 
      Efficient Drug Delivery System (DDS), Nanjing 210023, PR China.
FAU - Cai, Hao
AU  - Cai H
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China.
FAU - Li, Huaning
AU  - Li H
AD  - First Clinical Medical College, Nanjing Medical University, Nanjing 210029, PR 
      China.
FAU - Tao, Yi
AU  - Tao Y
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China.
FAU - Huang, Ping
AU  - Huang P
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China Jiangsu Provincial TCM Engineering Technology Research Center of High 
      Efficient Drug Delivery System (DDS), Nanjing 210023, PR China.
FAU - Qian, Xiaocui
AU  - Qian X
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China Jiangsu Provincial TCM Engineering Technology Research Center of High 
      Efficient Drug Delivery System (DDS), Nanjing 210023, PR China.
FAU - Li, Junsong
AU  - Li J
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China Jiangsu Provincial TCM Engineering Technology Research Center of High 
      Efficient Drug Delivery System (DDS), Nanjing 210023, PR China 
      lijunsong1964@163.com.
FAU - Cai, Baochang
AU  - Cai B
AD  - School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150922
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Formates)
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - 0YIW783RG1 (formic acid)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/methods/*standards
MH  - Drugs, Chinese Herbal
MH  - Flavonoids/*isolation & purification
MH  - Formates
MH  - Humans
MH  - Limit of Detection
MH  - Methanol
MH  - Plant Extracts/*chemistry
MH  - Reproducibility of Results
MH  - Scutellaria baicalensis/*chemistry
MH  - Solvents
MH  - Wine/*analysis
EDAT- 2015/09/25 06:00
MHDA- 2016/11/01 06:00
CRDT- 2015/09/25 06:00
PHST- 2014/12/15 00:00 [received]
PHST- 2015/09/25 06:00 [entrez]
PHST- 2015/09/25 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - bmv143 [pii]
AID - 10.1093/chromsci/bmv143 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2016 Mar;54(3):312-7. doi: 10.1093/chromsci/bmv143. Epub 2015 
      Sep 22.