PMID- 26401343
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150924
LR  - 20220317
IS  - 2052-6687 (Print)
IS  - 2052-6687 (Electronic)
IS  - 2052-6687 (Linking)
VI  - 2
DP  - 2015
TI  - A cultured approach to canine urothelial carcinoma: molecular characterization of 
      five cell lines.
PG  - 15
LID - 10.1186/s40575-015-0028-3 [doi]
LID - 15
AB  - BACKGROUND: Urothelial carcinoma (UC), also known as transitional cell carcinoma 
      (TCC), of the bladder is the most common neoplasm affecting the canine urogenital 
      system. To facilitate study of the disease in vitro, cell line models have been 
      established from primary tumor biopsies. Their resemblance to the primary 
      disease, however, has not been well defined. In the present study, we evaluated 
      five canine UC cell lines via oligonucleotide array comparative genomic 
      hybridization (oaCGH), fluorescence in situ hybridization (FISH), and gene 
      expression analysis. RESULTS: Comparison of genome wide DNA copy number profiles 
      of the cell lines with primary biopsy specimens revealed redundancies in genomic 
      aberrations, indicating that the cell lines retain the gross genomic architecture 
      of primary tumors. As in the primary tumors, gain of canine chromosomes 13 and 36 
      and loss of chromosome 19 were among the most frequent aberrations evident in the 
      cell lines. FISH analysis revealed chromosome structural aberrations, including 
      tandem duplications, bi-armed chromosomes, and chromosome fusions, suggesting 
      genome instability during neoplastic transformation. Gene expression profiling 
      highlighted numerous differentially expressed genes, including many previously 
      shown as dysregulated in primary canine UC and human bladder cancer. Pathway 
      enrichment analysis emphasized pathways suspected to be at the crux of UC 
      pathogenesis, including xenobiotic and lipid compound metabolism. CONCLUSIONS: 
      These data support valid use of the canine UC cell lines evaluated by confirming 
      they provide an accurate and practical means to interrogate the UC at a molecular 
      level. Moreover, the cell lines may provide a valuable model for furthering our 
      understanding of aberrant metabolic pathways in UC development.
FAU - Shapiro, S G
AU  - Shapiro SG
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607 USA.
FAU - Knapp, D W
AU  - Knapp DW
AD  - Department of Veterinary Clinical Sciences, Purdue University, College of 
      Veterinary Medicine, West Lafayette, IN USA ; Purdue University Center for Cancer 
      Research, West Lafayette, IN USA.
FAU - Breen, Matthew
AU  - Breen M
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607 USA 
      ; Center for Comparative Medicine and Translational Research, North Carolina 
      State University, Raleigh, NC USA ; Center for Human Health and the Environment, 
      North Carolina State University, Raleigh, NC USA ; Lineberger Comprehensive 
      Cancer Center, University of North Carolina, Chapel Hill, NC USA.
LA  - eng
GR  - T35 OD011070/OD/NIH HHS/United States
GR  - T35 RR025837/RR/NCRR NIH HHS/United States
PT  - Journal Article
DEP - 20150917
PL  - England
TA  - Canine Genet Epidemiol
JT  - Canine genetics and epidemiology
JID - 101664860
PMC - PMC4579363
OTO - NOTNLM
OT  - Bladder cancer
OT  - Cell lines
OT  - Chromosome
OT  - Lipid metabolism
OT  - Transitional cell carcinoma
OT  - Urothelial carcinoma
EDAT- 2015/09/25 06:00
MHDA- 2015/09/25 06:01
PMCR- 2015/09/17
CRDT- 2015/09/25 06:00
PHST- 2015/05/06 00:00 [received]
PHST- 2015/08/26 00:00 [accepted]
PHST- 2015/09/25 06:00 [entrez]
PHST- 2015/09/25 06:00 [pubmed]
PHST- 2015/09/25 06:01 [medline]
PHST- 2015/09/17 00:00 [pmc-release]
AID - 28 [pii]
AID - 10.1186/s40575-015-0028-3 [doi]
PST - epublish
SO  - Canine Genet Epidemiol. 2015 Sep 17;2:15. doi: 10.1186/s40575-015-0028-3. 
      eCollection 2015.