PMID- 26403239
OWN - NLM
STAT- MEDLINE
DCOM- 20160831
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 407
IP  - 29
DP  - 2015 Nov
TI  - In-line coupling of single-drop microextraction with capillary 
      electrophoresis-mass spectrometry.
PG  - 8745-52
LID - 10.1007/s00216-015-9028-0 [doi]
AB  - Single-drop microextraction (SDME) was in-line coupled with capillary 
      electrophoresis-mass spectrometry to provide sample cleanup and enrichment 
      simultaneously. Since there is no outlet vial in a conventional capillary 
      electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) 
      configuration, it is not easy to hang a single drop in the capillary inlet for 
      extraction. We overcame the difficulty of coupling SDME and CE-MS by using a 
      temporary outlet reservoir. Basic drugs such as methamphetamine, amphetamine, 
      phenethylamine, methoxyphenamine, and mephentermine were extracted from a basic 
      sample solution to an acidic acceptor drop covered with a thin octanol layer 
      formed at the capillary inlet tip. Compared to the CE-MS method in the multiple 
      reaction monitoring (MRM) mode, the in-line SDME-CE-MS/MS technique showed 
      130 approximately 150-fold enrichment in 10 min. The relative standard deviations (RSDs) of 
      peak height ranged from 9 to 13 %. RSDs can be reduced from 4 to 6 % using 
      mephentermine as an internal standard. We examined the pretreatment of sample 
      with and without SDME from human urine under the full-scan mode, which confirmed 
      that many metabolites were cleaned up by the selective extraction method of SDME. 
      Even if the analytes from human urine were analyzed under the MRM mode used as a 
      mass filter, there was an isobaric compound causing a disturbance to the 
      analysis. However, in-line SDME-CE-MS/MS made it possible to perform a sample 
      cleanup as well as sample enrichment. The research is extremely advantageous in 
      that it is rapid, convenient, and highly sensitive for the analysis of biological 
      samples using a commercially available instrument.
FAU - Kim, Jihye
AU  - Kim J
AD  - Department of Chemistry, Seoul National University, Seoul, 151-747, South Korea.
FAU - Choi, Kihwan
AU  - Choi K
AD  - Division of Methodology for Quality of Life, Korea Research Institute of 
      Standards and Science, Daejeon, 305-340, South Korea.
FAU - Chung, Doo Soo
AU  - Chung DS
AD  - Department of Chemistry, Seoul National University, Seoul, 151-747, South Korea. 
      dschung@snu.ac.kr.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150924
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Pharmaceutical Preparations)
SB  - IM
MH  - Drug Monitoring/instrumentation
MH  - Electrophoresis, Capillary/*instrumentation
MH  - Equipment Design
MH  - Humans
MH  - Limit of Detection
MH  - Liquid Phase Microextraction/*instrumentation
MH  - Pharmaceutical Preparations/*urine
MH  - Spectrometry, Mass, Electrospray Ionization/*instrumentation
OTO - NOTNLM
OT  - Capillary electrophoresis-mass spectrometry (CE-MS)
OT  - Drug analysis
OT  - In-line coupling
OT  - Sample preparation
OT  - Single-drop microextraction (SDME)
OT  - Urine
EDAT- 2015/09/26 06:00
MHDA- 2016/09/01 06:00
CRDT- 2015/09/26 06:00
PHST- 2015/07/12 00:00 [received]
PHST- 2015/09/03 00:00 [accepted]
PHST- 2015/08/10 00:00 [revised]
PHST- 2015/09/26 06:00 [entrez]
PHST- 2015/09/26 06:00 [pubmed]
PHST- 2016/09/01 06:00 [medline]
AID - 10.1007/s00216-015-9028-0 [pii]
AID - 10.1007/s00216-015-9028-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2015 Nov;407(29):8745-52. doi: 10.1007/s00216-015-9028-0. Epub 
      2015 Sep 24.