PMID- 26403275 OWN - NLM STAT- MEDLINE DCOM- 20160818 LR - 20181113 IS - 1365-2826 (Electronic) IS - 0953-8194 (Print) IS - 0953-8194 (Linking) VI - 27 IP - 11 DP - 2015 Nov TI - Localisation of 11beta-Hydroxysteroid Dehydrogenase Type 2 in Mineralocorticoid Receptor Expressing Magnocellular Neurosecretory Neurones of the Rat Supraoptic and Paraventricular Nuclei. PG - 835-49 LID - 10.1111/jne.12325 [doi] AB - An accumulating body of evidence suggests that the activity of the mineralocorticoid, aldosterone, in the brain via the mineralocorticoid receptor (MR) plays an important role in the regulation of blood pressure. MR was recently found in vasopressin and oxytocin synthesising magnocellular neurosecretory cells (MNCs) in both the paraventricular (PVN) and supraoptic (SON) nuclei in the hypothalamus. Considering the physiological effects of these hormones, MR in these neurones may be an important site mediating the action of aldosterone in blood pressure regulation within the brain. However, aldosterone activation of MR in the hypothalamus remains controversial as a result of the high binding affinity of glucocorticoids to MR at substantially higher concentrations compared to aldosterone. In aldosterone-sensitive epithelia, the enzyme 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) prevents glucocorticoids from binding to MR by converting glucocorticoids into inactive metabolites. The present study aimed to determine whether 11beta-HSD2, which increases aldosterone selectivity, is expressed in MNCs. Specific 11beta-HSD2 immunoreactivity was found in the cytoplasm of the MNCs in both the SON and PVN. In addition, double-fluorescence confocal microscopy demonstrated that MR-immunoreactivity and 11beta-HSD2-in situ hybridised products are colocalised in MNCs. Lastly, single-cell reverse transcriptase-polymerase chain reaction detected MR and 11beta-HSD2 mRNAs from cDNA libraries derived from single identified MNCs. These findings strongly suggest that MNCs in the SON and PVN are aldosterone-sensitive neurones. CI - (c) 2015 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology. FAU - Haque, M AU - Haque M AD - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA. FAU - Wilson, R AU - Wilson R AD - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA. FAU - Sharma, K AU - Sharma K AD - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA. FAU - Mills, N J AU - Mills NJ AD - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA. FAU - Teruyama, R AU - Teruyama R AD - Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA. LA - eng GR - R01 HL115208/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PL - United States TA - J Neuroendocrinol JT - Journal of neuroendocrinology JID - 8913461 RN - 0 (Receptors, Mineralocorticoid) RN - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 2) SB - IM MH - 11-beta-Hydroxysteroid Dehydrogenase Type 2/*metabolism MH - Animals MH - Male MH - Neurosecretory Systems/*cytology/enzymology/*metabolism MH - Paraventricular Hypothalamic Nucleus/*cytology/enzymology/metabolism MH - Rats MH - Receptors, Mineralocorticoid/*metabolism MH - Supraoptic Nucleus/*cytology/enzymology/metabolism PMC - PMC5019266 OTO - NOTNLM OT - oxytocin OT - vasopressin EDAT- 2015/09/26 06:00 MHDA- 2016/08/19 06:00 PMCR- 2016/09/12 CRDT- 2015/09/26 06:00 PHST- 2015/06/01 00:00 [received] PHST- 2015/09/15 00:00 [revised] PHST- 2015/09/17 00:00 [accepted] PHST- 2015/09/26 06:00 [entrez] PHST- 2015/09/26 06:00 [pubmed] PHST- 2016/08/19 06:00 [medline] PHST- 2016/09/12 00:00 [pmc-release] AID - JNE12325 [pii] AID - 10.1111/jne.12325 [doi] PST - ppublish SO - J Neuroendocrinol. 2015 Nov;27(11):835-49. doi: 10.1111/jne.12325.