PMID- 26405983
OWN - NLM
STAT- MEDLINE
DCOM- 20160722
LR  - 20150926
IS  - 1878-3619 (Electronic)
IS  - 0959-2989 (Linking)
VI  - 26 Suppl 1
DP  - 2015
TI  - Inhibition of RhoA expression by adenovirus-mediated siRNA combined with TNF-alpha 
      induced apoptosis of hepatocarcinoma cells.
PG  - S2055-67
LID - 10.3233/BME-151511 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been used as an effective treatment for 
      Hepatocellular Carcinoma, however, inducing tumor cell apoptosis by TNF-alpha alone 
      is still unsatisfactory. RhoA is highly expressed in hepatocarcinoma cells and 
      can be activated by TNF-alpha. The activation of RhoA directly leads to a poor 
      prognosis of HCC. Therefore, we propose to investigate the therapeutic effect of 
      TNF-alpha together with RhoA siRNA. RhoA inhibition was accomplished by constructing 
      a recombinant adenovirus that can efficiently express RhoA siRNA in HepG2 cells. 
      The recombinant adenovirus AdshRNA-RhoA and AdU6-control were generated by 
      adenovirus-mediated siRNA expression system. The inhibition effects were detected 
      by RT-PCR in addition to immunoblot to quantify the decreased levels of RhoA 
      expression, and the therapeutic effect for HCC was demonstrated by the 
      proliferation and apoptosis ratios of HepG2 cells. The inhibition effects of RhoA 
      by AdshRNA-RhoA were significant at both mRNA and protein levels: the 
      transcription of RhoA mRNA decreased by 74.46%, and the expression of protein 
      decreased by 76.48%. The proliferation rate of HepG2 cells detected by MTT showed 
      that a treatment of AdshRNA-RhoA and TNF-alpha together could strengthen the 
      suppression ability of TNF-alpha to HepG2 cells, resulting in approximately 14.2% 
      more than those treated with only TNF-alpha. FCA and TUNEL assays results revealed 
      that the combined treatment can induce apoptosis in approximately 52.14%-65% of 
      the HepG2 cells, whereas this ratio in the TNF-alpha-alone group was only 21.91%-32%. 
      Our results showed that AdshRNA-RhoA can efficiently enhance the TNF-alpha-induced 
      apoptosis of hepatocarcinoma cells. This method might be a useful therapeutic 
      route in HCC and other tumors.
FAU - Yin, Kun
AU  - Yin K
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Zhao, Guihua
AU  - Zhao G
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Huang, Xiaodan
AU  - Huang X
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Gao, Ge
AU  - Gao G
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Sun, Hui
AU  - Sun H
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Wei, Qingkuan
AU  - Wei Q
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Liu, Qiaoqiao
AU  - Liu Q
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Li, Mian
AU  - Li M
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Xu, Chao
AU  - Xu C
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Zhu, Song
AU  - Zhu S
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
FAU - Ba, Zaihua
AU  - Ba Z
AD  - Jining Medical University, 16 Hehua Road, Jining 272000, China.
FAU - Yan, Ge
AU  - Yan G
AD  - Shandong Academy of Medical Sciences, Shandong Institute of Parasitical Disease, 
      11 Taibai Middle Road, Jining 272033, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomed Mater Eng
JT  - Bio-medical materials and engineering
JID - 9104021
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Apoptosis
MH  - Carcinoma, Hepatocellular/genetics/pathology/*therapy
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver/metabolism/pathology
MH  - Liver Neoplasms/genetics/pathology/*therapy
MH  - RNA, Small Interfering/genetics/*therapeutic use
MH  - *RNAi Therapeutics
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - rhoA GTP-Binding Protein/*genetics
OTO - NOTNLM
OT  - RhoA
OT  - adenovirus-mediated siRNA
OT  - apoptosis
OT  - hepatocarcinoma cells
OT  - tumor necrosis factor-alpha
EDAT- 2015/09/26 06:00
MHDA- 2016/07/23 06:00
CRDT- 2015/09/26 06:00
PHST- 2015/09/26 06:00 [entrez]
PHST- 2015/09/26 06:00 [pubmed]
PHST- 2016/07/23 06:00 [medline]
AID - BME1511 [pii]
AID - 10.3233/BME-151511 [doi]
PST - ppublish
SO  - Biomed Mater Eng. 2015;26 Suppl 1:S2055-67. doi: 10.3233/BME-151511.