PMID- 26407639
OWN - NLM
STAT- MEDLINE
DCOM- 20160317
LR  - 20220409
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 575
IP  - 2 Pt 3
DP  - 2016 Jan 10
TI  - Identification of cold responsive genes in Pacific white shrimp (Litopenaeus 
      vannamei) by suppression subtractive hybridization.
PG  - 667-74
LID - S0378-1119(15)01154-3 [pii]
LID - 10.1016/j.gene.2015.09.045 [doi]
AB  - The Pacific white shrimp (Litopenaeus vannamei) is one of the most widely 
      cultured shrimp species in the world. Despite L. vannamei having tropical 
      origins, it is being reared subtropically, with low temperature stress being one 
      of the most severe threats to its growth, survival and distribution. To unravel 
      the molecular basis of cold tolerance in L. vannamei, the suppression subtractive 
      hybridization (SSH) platform was employed to identify cold responsive genes in 
      the hepatopancreas of L. vannamei. Both forward and reverse cDNA libraries were 
      constructed, followed by dot blot hybridization, cloning, sequence analysis and 
      quantitative real-time PCR. These approaches identified 92 cold induced and 48 
      cold inhibited ESTs to give a total of 37 cold induced and 17 cold inhibited 
      contigs. Some of the identified genes related to stress response or cell defense, 
      such as tetraspanins (TSPANs), DEAD-box helicase, heat shock proteins (HSPs) and 
      metallothionein (MT), which were more abundant in the forward SSH library than in 
      the reverse SSH library. The most abundant Est was a tetraspanin-8 (TSPAN8) 
      homolog dubbed LvTSPAN8. A multiple sequence alignment and transmembrane domain 
      prediction was also performed for LvTSPAN8. LvTSPAN8 expression was also examined 
      in the gills, muscle, heart and hepatopancreas following cold exposure and showed 
      the highest expression levels in the hepatopancreas. Overall, this study was able 
      to identify several known genes and novel genes via SSH that appear to be 
      associated with cold stress and will help to provide further insights into the 
      molecular mechanisms regulating cold tolerance in L. vannamei.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Peng, Jinxia
AU  - Peng J
AD  - Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, 
      Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
FAU - Wei, Pinyuan
AU  - Wei P
AD  - Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, 
      Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
FAU - Chen, Xiuli
AU  - Chen X
AD  - Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, 
      Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
FAU - Zeng, Digang
AU  - Zeng D
AD  - Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, 
      Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
FAU - Chen, Xiaohan
AU  - Chen X
AD  - Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, 
      Academy of Fishery Sciences, Nanning, Guangxi 530021, China. Electronic address: 
      chnxhn@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150925
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Arthropod Proteins)
SB  - IM
MH  - Animals
MH  - Arthropod Proteins/*genetics
MH  - Cold Temperature
MH  - Expressed Sequence Tags
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation
MH  - Hepatopancreas/metabolism
MH  - Penaeidae/*genetics
MH  - *Stress, Physiological
MH  - Subtractive Hybridization Techniques/*methods
MH  - Tissue Distribution
OTO - NOTNLM
OT  - Cold tolerance
OT  - Litopenaeus vannamei
OT  - SSH
OT  - Tetraspanin
EDAT- 2015/09/27 06:00
MHDA- 2016/03/18 06:00
CRDT- 2015/09/27 06:00
PHST- 2015/06/03 00:00 [received]
PHST- 2015/08/28 00:00 [revised]
PHST- 2015/09/18 00:00 [accepted]
PHST- 2015/09/27 06:00 [entrez]
PHST- 2015/09/27 06:00 [pubmed]
PHST- 2016/03/18 06:00 [medline]
AID - S0378-1119(15)01154-3 [pii]
AID - 10.1016/j.gene.2015.09.045 [doi]
PST - ppublish
SO  - Gene. 2016 Jan 10;575(2 Pt 3):667-74. doi: 10.1016/j.gene.2015.09.045. Epub 2015 
      Sep 25.