PMID- 26408985 OWN - NLM STAT- MEDLINE DCOM- 20160503 LR - 20181202 IS - 1872-7786 (Electronic) IS - 0009-2797 (Linking) VI - 242 DP - 2015 Dec 5 TI - N-palmitoyl serotonin alleviates scopolamine-induced memory impairment via regulation of cholinergic and antioxidant systems, and expression of BDNF and p-CREB in mice. PG - 153-62 LID - S0009-2797(15)30069-7 [pii] LID - 10.1016/j.cbi.2015.09.016 [doi] AB - N-Palmitoyl-5-hydroxytryptamines (Pal-5HT), a cannabinoid, has recently been reported to express anti-allergic and anti-inflammatory actions in RBL-2H3 cells, and ameliorate glutamate-induced cytotoxicity in HT-22 cells. In this study, we examined the effect of Pal-5HT on deficits of learning and memory induced by scopolamine in mice. Memory performance was evaluated using Morris water maze test and passive avoidance test. Activities of acetylcholinesterase (AChE) and choline acetyltransferase (ChAT), level of oxidative stress markers, and expression of brain-derived neurotrophic factor (BDNF), phosphorylation of cAMP response element-binding protein (p-CREB) were determined. Loss of neuronal cells in hippocampus was evaluated by histological examinations. Pal-5HT significantly improved the amnesia in the behavioral assessment. Pal-5HT regulated cholinergic function by inhibiting scopolamine-induced elevation of AChE activity and decline of ChAT activity. Pal-5HT suppressed oxidative stress by increasing activities of glutathione peroxidase (GPx), glutathione reductase (GR) or NAD(P)H quinine oxidoreductase-1 (NQO-1) and lowering MDA level. Additionally, it prevented against scopolamine-induced expression of iNOS and COX-2. Moreover, Pal-5HT suppressed the death of neuronal cells in CA1 and CA3 regions, while it restored expression of p-CREB and BDNF in hippocampus. Taken together, Pal-5HT is suggested to ameliorate deficits of memory and learning through regulation of cholinergic function, activation of antioxidant systems as well as restoration of BDNF and p-CREB expression. From these, Pal-5HT may be a potential candidate to prevent against neurodegeneration related to the memory deficit. CI - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved. FAU - Min, A Young AU - Min AY AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. FAU - Doo, Choon Nan AU - Doo CN AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. FAU - Son, Eun Jung AU - Son EJ AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. FAU - Sung, Nak Yun AU - Sung NY AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. FAU - Lee, Kun Jong AU - Lee KJ AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. FAU - Sok, Dai-Eun AU - Sok DE AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. FAU - Kim, Mee Ree AU - Kim MR AD - Department of Food and Nutrition, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: mrkim@cnu.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150925 PL - Ireland TA - Chem Biol Interact JT - Chemico-biological interactions JID - 0227276 RN - 0 (Antioxidants) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Creb1 protein, mouse) RN - 0 (Cyclic AMP Response Element-Binding Protein) RN - 333DO1RDJY (Serotonin) RN - DL48G20X8X (Scopolamine) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type II) RN - EC 1.14.13.39 (Nos2 protein, mouse) RN - EC 1.14.99.- (Ptgs2 protein, mouse) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone)) RN - EC 1.6.5.2 (Nqo1 protein, mouse) RN - EC 2.3.1.6 (Choline O-Acetyltransferase) RN - EC 3.1.1.7 (Acetylcholinesterase) SB - IM MH - Acetylcholinesterase/metabolism MH - Animals MH - Antioxidants/metabolism MH - Avoidance Learning/drug effects MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Choline O-Acetyltransferase/metabolism MH - Cyclic AMP Response Element-Binding Protein/*metabolism MH - Cyclooxygenase 2/metabolism MH - Hippocampus/drug effects/metabolism MH - Male MH - Maze Learning/drug effects MH - Memory Disorders/chemically induced/*drug therapy MH - Mice, Inbred ICR MH - NAD(P)H Dehydrogenase (Quinone)/metabolism MH - Nitric Oxide Synthase Type II/metabolism MH - Scopolamine/*adverse effects MH - Serotonin/*analogs & derivatives/pharmacology OTO - NOTNLM OT - Antioxidant system OT - BDNF OT - Cholinergic function OT - Pal-5HT OT - Scopolamine OT - p-CREB EDAT- 2015/09/27 06:00 MHDA- 2016/05/04 06:00 CRDT- 2015/09/27 06:00 PHST- 2015/05/06 00:00 [received] PHST- 2015/09/10 00:00 [revised] PHST- 2015/09/17 00:00 [accepted] PHST- 2015/09/27 06:00 [entrez] PHST- 2015/09/27 06:00 [pubmed] PHST- 2016/05/04 06:00 [medline] AID - S0009-2797(15)30069-7 [pii] AID - 10.1016/j.cbi.2015.09.016 [doi] PST - ppublish SO - Chem Biol Interact. 2015 Dec 5;242:153-62. doi: 10.1016/j.cbi.2015.09.016. Epub 2015 Sep 25.