PMID- 26410104
OWN - NLM
STAT- MEDLINE
DCOM- 20160427
LR  - 20151215
IS  - 1872-7905 (Electronic)
IS  - 0022-1759 (Linking)
VI  - 427
DP  - 2015 Dec
TI  - Human immune system mice immunized with Plasmodium falciparum circumsporozoite 
      protein induce protective human humoral immunity against malaria.
PG  - 42-50
LID - S0022-1759(15)30047-8 [pii]
LID - 10.1016/j.jim.2015.09.005 [doi]
AB  - In this study, we developed human immune system (HIS) mice that possess 
      functional human CD4+ T cells and B cells, named HIS-CD4/B mice. HIS-CD4/B mice 
      were generated by first introducing HLA class II genes, including DR1 and DR4, 
      along with genes encoding various human cytokines and human B cell activation 
      factor (BAFF) to NSG mice by adeno-associated virus serotype 9 (AAV9) vectors, 
      followed by engrafting human hematopoietic stem cells (HSCs). HIS-CD4/B mice, in 
      which the reconstitution of human CD4+ T and B cells resembles to that of humans, 
      produced a significant level of human IgG against Plasmodium falciparum 
      circumsporozoite (PfCS) protein upon immunization. CD4+ T cells in HIS-CD4/B 
      mice, which possess central and effector memory phenotypes like those in humans, 
      are functional, since PfCS protein-specific human CD4+ T cells secreting IFN-gamma 
      and IL-2 were detected in immunized HIS-CD4/B mice. Lastly, PfCS 
      protein-immunized HIS-CD4/B mice were protected from in vivo challenge with 
      transgenic P. berghei sporozoites expressing the PfCS protein. The immune sera 
      collected from protected HIS-CD4/B mice reacted against transgenic P. berghei 
      sporozoites expressing the PfCS protein and also inhibited the parasite invasion 
      into hepatocytes in vitro. Taken together, these studies show that our HIS-CD4/B 
      mice could mount protective human anti-malaria immunity, consisting of human IgG 
      and human CD4+ T cell responses both specific for a human malaria antigen.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Huang, Jing
AU  - Huang J
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA.
FAU - Li, Xiangming
AU  - Li X
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA.
FAU - Coelho-dos-Reis, Jordana G A
AU  - Coelho-dos-Reis JG
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA.
FAU - Zhang, Min
AU  - Zhang M
AD  - Department of Pathology, New York University School of Medicine, New York, NY, 
      USA.
FAU - Mitchell, Robert
AU  - Mitchell R
AD  - Division of Parasitology, Department of Microbiology, New York University School 
      of Medicine, New York, NY, USA.
FAU - Nogueira, Raquel Tayar
AU  - Nogueira RT
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA.
FAU - Tsao, Tiffany
AU  - Tsao T
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA.
FAU - Noe, Amy R
AU  - Noe AR
AD  - Leidos, San Diego, CA, USA.
FAU - Ayala, Ramses
AU  - Ayala R
AD  - Leidos, Frederick, MD, USA.
FAU - Sahi, Vincent
AU  - Sahi V
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA.
FAU - Gutierrez, Gabriel M
AU  - Gutierrez GM
AD  - Leidos, Frederick, MD, USA.
FAU - Nussenzweig, Victor
AU  - Nussenzweig V
AD  - Department of Pathology, New York University School of Medicine, New York, NY, 
      USA.
FAU - Wilson, James M
AU  - Wilson JM
AD  - Gene Therapy Program, Department of Pathology and Laboratory Medicine, University 
      of Pennsylvania, Philadelphia, PA, USA.
FAU - Nardin, Elizabeth H
AU  - Nardin EH
AD  - Division of Parasitology, Department of Microbiology, New York University School 
      of Medicine, New York, NY, USA.
FAU - Nussenzweig, Ruth S
AU  - Nussenzweig RS
AD  - Division of Parasitology, Department of Microbiology, New York University School 
      of Medicine, New York, NY, USA.
FAU - Tsuji, Moriya
AU  - Tsuji M
AD  - Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New 
      York, NY, USA. Electronic address: mtsuji@adarc.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150926
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Malaria Vaccines)
RN  - 0 (Protozoan Proteins)
RN  - 0 (circumsporozoite protein, Protozoan)
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Heterografts
MH  - Histocompatibility Antigens Class II
MH  - Humans
MH  - Immunity, Humoral/*immunology
MH  - Malaria Vaccines
MH  - Malaria, Falciparum/*immunology
MH  - Mice
MH  - Mice, Transgenic/*immunology
MH  - Protozoan Proteins/immunology
OTO - NOTNLM
OT  - HLA class II molecule
OT  - Human IgG
OT  - Human immune system mice
OT  - P. falciparum circumsporozoite protein
OT  - Protective anti-malaria immunity
EDAT- 2015/09/28 06:00
MHDA- 2016/04/28 06:00
CRDT- 2015/09/28 06:00
PHST- 2015/05/15 00:00 [received]
PHST- 2015/08/17 00:00 [revised]
PHST- 2015/09/23 00:00 [accepted]
PHST- 2015/09/28 06:00 [entrez]
PHST- 2015/09/28 06:00 [pubmed]
PHST- 2016/04/28 06:00 [medline]
AID - S0022-1759(15)30047-8 [pii]
AID - 10.1016/j.jim.2015.09.005 [doi]
PST - ppublish
SO  - J Immunol Methods. 2015 Dec;427:42-50. doi: 10.1016/j.jim.2015.09.005. Epub 2015 
      Sep 26.