PMID- 26410998
OWN - NLM
STAT- MEDLINE
DCOM- 20151116
LR  - 20150928
IS  - 1934-578X (Print)
IS  - 1555-9475 (Linking)
VI  - 10
IP  - 7
DP  - 2015 Jul
TI  - Preferentially Cytotoxic Constituents of Andrographis paniculata and their 
      Preferential Cytotoxicity against Human Pancreatic Cancer Cell Lines.
PG  - 1153-8
AB  - In the course of our search for anticancer agents based on a novel anti-austerity 
      strategy, we found that the 70% EtOH extract of the crude drug Andrographis Herba 
      (aerial parts of Andrographis paniculata), used in Japanese Kampo medicines, 
      killed PANC-1 human pancreatic cancer cells preferentially in nutrient-deprived 
      medium (NDM). Phytochemical investigation of the 70% EtOH extract led to the 
      isolation of 21 known compounds consisting of six labdane-type diterpenes (11, 
      15, 17-19, 21), six flavones (5, 7, 10, 12, 14, 20), three flavanones (2, 6, 16), 
      two sterols (3, 8), a fatty acid (1), a phthalate (4), a triterpene (9), and a 
      monoterpene (13). Among them, 14-deoxy-11,12-didehydroandrographolide (17) 
      displayed the most potent preferential cytotoxicity against PANC-1 and PSN-1 
      cells with PC50 values of 10.0 muM and 9.27 muM, respectively. Microscopical 
      observation, double staining with ethidium bromide (EB) and acridine orange (AO), 
      and flow cytometry with propidium iodide/annexin V double staining indicated that 
      14-deoxy-11,12-didehydroandrographolide (17) triggered apoptosis-like cell death 
      in NDM with an amino acids and/or serum-sensitive mode.
FAU - Lee, Sullim
AU  - Lee S
FAU - Morita, Hiroyuki
AU  - Morita H
FAU - Tezuka, Yasuhiro
AU  - Tezuka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Prod Commun
JT  - Natural product communications
JID - 101477873
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Diterpenes)
RN  - 0 (Flavonoids)
RN  - 0 (Phytosterols)
SB  - IM
MH  - Andrographis/*chemistry
MH  - Antineoplastic Agents, Phytogenic/*isolation & purification
MH  - Cell Line, Tumor
MH  - Diterpenes/isolation & purification
MH  - Drug Screening Assays, Antitumor
MH  - Flavonoids/isolation & purification
MH  - Humans
MH  - Phytosterols/isolation & purification
EDAT- 2015/09/29 06:00
MHDA- 2015/11/17 06:00
CRDT- 2015/09/29 06:00
PHST- 2015/09/29 06:00 [entrez]
PHST- 2015/09/29 06:00 [pubmed]
PHST- 2015/11/17 06:00 [medline]
PST - ppublish
SO  - Nat Prod Commun. 2015 Jul;10(7):1153-8.