PMID- 26411784
OWN - NLM
STAT- MEDLINE
DCOM- 20160819
LR  - 20181113
IS  - 1536-2442 (Electronic)
IS  - 1536-2442 (Linking)
VI  - 15
IP  - 1
DP  - 2015
TI  - Analysis of Inter-Individual Bacterial Variation in Gut of Cicada Meimuna 
      mongolica (Hemiptera: Cicadidae).
LID - 10.1093/jisesa/iev113 [doi]
LID - 131
AB  - Intestinal bacterial community plays a crucial role in the nutrition, 
      development, survival, and reproduction of insects. When compared with other 
      insects with piercing-sucking mouthparts, the habitats of cicada nymphs and 
      adults are totally different. However, little is known about the differences in 
      the gut bacterial communities in the nymphs and adults within any cicada species. 
      The diversity of bacteria in the gut of nymphs and adults of both genders of 
      Meimuna mongolica (Distant) was studied using the denaturing gradient gel 
      electrophoresis (DGGE) method. Few inter-individual variations among gut 
      microbiota were observed, suggesting that M. mongolica typically harbors a 
      limited and consistent suite of bacterial species. Bacteria in the genera 
      Pseudomonas and Enterobacter were the predominant components of the gut 
      microflora of M. mongolica at all life stages. Bacteria of Pantoea, 
      Streptococcus, and Uruburuella were also widespread in the cicada samples but at 
      relatively lower concentrations. The relative stability and similarity of the 
      PCR-DGGE patterns indicate that all individuals of this cicada species harbor a 
      characteristic bacterial community which is independent from developmental stages 
      and genders. Related endosymbionts that could be harbored in bacteromes of 
      cicadas were not detected in any gut samples, which could be related to the 
      cicada species and the distribution of these endosymbionts in the cicada cavity, 
      or due to some of the possible limitations of PCR-DGGE community profiling. It is 
      worthwhile to further address if related cicada endosymbiont clades distribute in 
      the alimentary canals and other internal organs through diagnostic PCR using 
      group-specific primer sets.
CI  - (c) The Author 2015. Published by Oxford University Press on behalf of the 
      Entomological Society of America.
FAU - Zhou, Wenting
AU  - Zhou W
AD  - Key Laboratory of Plant Protection Resources and Pest Management, Ministry of 
      Education, Entomological Museum, China *These authors contributed equally to this 
      work.
FAU - Nan, Xiaoning
AU  - Nan X
AD  - College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China 
      *These authors contributed equally to this work.
FAU - Zheng, Zhou
AU  - Zheng Z
AD  - Key Laboratory of Plant Protection Resources and Pest Management, Ministry of 
      Education, Entomological Museum, China.
FAU - Wei, Cong
AU  - Wei C
AD  - Key Laboratory of Plant Protection Resources and Pest Management, Ministry of 
      Education, Entomological Museum, China.
FAU - He, Hong
AU  - He H
AD  - College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China 
      hehong@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150927
PL  - United States
TA  - J Insect Sci
JT  - Journal of insect science (Online)
JID - 101096396
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
EIN - J Insect Sci. 2016;16. pii: 23. doi: 10.1093/jisesa/iew004. PMID: 26903495
MH  - Animals
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - Female
MH  - Gastrointestinal Tract/*microbiology
MH  - Hemiptera/*microbiology
MH  - Male
MH  - Nymph/microbiology
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
PMC - PMC4626675
OTO - NOTNLM
OT  - Meimuna mongolica
OT  - PCR-DGGE
OT  - characteristic bacterial community
OT  - gut bacteria
OT  - inter-individual variation
EDAT- 2015/09/29 06:00
MHDA- 2016/08/20 06:00
PMCR- 2015/01/01
CRDT- 2015/09/29 06:00
PHST- 2014/11/30 00:00 [received]
PHST- 2015/08/31 00:00 [accepted]
PHST- 2015/09/29 06:00 [entrez]
PHST- 2015/09/29 06:00 [pubmed]
PHST- 2016/08/20 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - iev113 [pii]
AID - 10.1093/jisesa/iev113 [doi]
PST - epublish
SO  - J Insect Sci. 2015 Sep 27;15(1):131. doi: 10.1093/jisesa/iev113. Print 2015.