PMID- 26414643
OWN - NLM
STAT- MEDLINE
DCOM- 20161025
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 24
IP  - 24
DP  - 2015 Dec 15
TI  - MicroRNA-498 Inhibition Enhances the Differentiation of Human Adipose-Derived 
      Mesenchymal Stem Cells into Podocyte-Like Cells.
PG  - 2841-52
LID - 10.1089/scd.2015.0027 [doi]
AB  - Podocyte depletion is a key event in the progression of end-stage kidney disease 
      (ESKD) resulting in nephrotic proteinuria and renal failure, but the treatment 
      options are limited to dialysis and renal transplantation. So there is an urgent 
      need for renal regenerative therapies. Generation of podocytes from human stem 
      cells is regarded as a promising therapeutic strategy to repair or regenerate the 
      damaged kidneys; however, the reliable induction system remains a challenge. In 
      this study, we established a two-stage induction protocol for podocyte generation 
      from human adipose-derived mesenchymal stem cells (hAD-MSCs). We initially 
      established a condition that induces hAD-MSCs toward intermediate mesoderm cells 
      with activin A and high concentration of retinoic acid (RA). Subsequently, by 
      using the combination of activin A and low concentration of RA and BMP7, we 
      generated podocyte-like cells expressing multiple podocyte-specific markers and 
      able to integrate into a developing nephron of embryonic kidney explant culture 
      and ameliorate proteinuria and kidney injure in adriamycin-treated mice. 
      Furthermore, we identified that miRNA-498 inhibitor has potential to improve the 
      differentiation of hAD-MSCs into podocyte-like cells and established a robust 
      induction protocol. Thereby, our study advocated an efficient method for the 
      induction of kidney podocyte-like (iPod) cells from hAD-MSCs and provided an 
      ideal candidate for regenerative therapies of the kidney.
FAU - Zhang, Lina
AU  - Zhang L
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
FAU - Li, Kanghua
AU  - Li K
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
FAU - Yan, Xi
AU  - Yan X
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
FAU - Liang, Xiaolei
AU  - Liang X
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
FAU - Wang, Shihua
AU  - Wang S
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
FAU - Han, Qin
AU  - Han Q
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
FAU - Zhao, Robert Chunhua
AU  - Zhao RC
AD  - 1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School 
      of Basic Medicine Peking Union Medical College , Beijing, People's Republic of 
      China .
AD  - 2 Peking Union Medical College Hospital , Beijing, People's Republic of China .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151026
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (MIRN498 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (activin A)
RN  - 104625-48-1 (Activins)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Activins/pharmacology
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Bone Morphogenetic Protein 7/pharmacology
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Humans
MH  - Kidney/cytology/physiology
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*antagonists & inhibitors
MH  - Podocytes/*cytology/metabolism
MH  - Regeneration
MH  - Tretinoin/pharmacology
EDAT- 2015/09/29 06:00
MHDA- 2016/10/26 06:00
CRDT- 2015/09/29 06:00
PHST- 2015/09/29 06:00 [entrez]
PHST- 2015/09/29 06:00 [pubmed]
PHST- 2016/10/26 06:00 [medline]
AID - 10.1089/scd.2015.0027 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2015 Dec 15;24(24):2841-52. doi: 10.1089/scd.2015.0027. Epub 2015 
      Oct 26.