PMID- 26416064
OWN - NLM
STAT- MEDLINE
DCOM- 20151112
LR  - 20150929
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 20
IP  - 4
DP  - 2015 Jul-Aug
TI  - Inhibition of tumor necrosis factor alpha using RNA interference in oral squamous 
      cell carcinoma.
PG  - 1107-14
AB  - PURPOSE: Oral squamous cell carcinoma (OSCC) is a disease with increased 
      prevalence and unfavorable prognosis calling for development of novel therapeutic 
      strategies. Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine 
      implicated in the development and progression of cancer. The present study was 
      designed to assess the impact of TNF-alpha specific inhibition using small 
      interference RNA (siRNA) in SSC-4 cells, a representative model for OSCC. 
      METHODS: The present study evaluated the effect of TNF-alpha inhibition using siRNA 
      as inhibitory mechanism on SCC-4 cells. The study focused on the effect of TNF-alpha 
      inhibition on apoptosis, autophagy and invasion in parallel with a panel of 20 
      genes involved in apoptosis and angiogenesis. RESULTS: TNF-alpha inhibition was 
      related with reduction of cell viability, activation of apoptosis and autophagy 
      in parallel with the inhibition of migration in SCC-4 cells. Evaluating the 
      impact on gene expression levels, inhibition of FASL-FADD, NFkappaB, SEMA 3C, TNF-alpha, 
      TGFB1, VEGFA, along with activation of PDGFB and SEMA 3D was observed. Our study 
      confirms the important role of TNF-alpha and sustains that it might be a therapeutic 
      target in OSCC. CONCLUSIONS: TNF-alpha is a key mediator of the immune system, with 
      important role in OSCC tumorigenesis, and might be considered as a therapeutic 
      target using siRNA technology, particularly for those risk cases having FASL/FADD 
      overexpressed.
FAU - Iulia Irimie, Alexandra
AU  - Iulia Irimie A
AD  - Department of Dental Prosthetics and Dental Materials, Faculty of Dental 
      Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, 
      Romania.
FAU - Braicu, Cornelia
AU  - Braicu C
FAU - Zanoaga, Oana
AU  - Zanoaga O
FAU - Pileczki, Valentina
AU  - Pileczki V
FAU - Soritau, Olga
AU  - Soritau O
FAU - Berindan-Neagoe, Ioana
AU  - Berindan-Neagoe I
FAU - Septimiu Campian, Radu
AU  - Septimiu Campian R
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Apoptosis
MH  - Autophagy
MH  - Carcinoma, Squamous Cell/pathology/*therapy
MH  - Cell Line, Tumor
MH  - Epistasis, Genetic
MH  - Humans
MH  - Mouth Neoplasms/pathology/*therapy
MH  - *RNA Interference
MH  - Transforming Growth Factor beta1/physiology
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors
EDAT- 2015/09/30 06:00
MHDA- 2015/11/13 06:00
CRDT- 2015/09/30 06:00
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2015/11/13 06:00 [medline]
PST - ppublish
SO  - J BUON. 2015 Jul-Aug;20(4):1107-14.