PMID- 26416423
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20181113
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 6
IP  - 31
DP  - 2015 Oct 13
TI  - Positron emission tomography imaging of cardiomyocyte apoptosis with a novel 
      molecule probe [18F]FP-DPAZn2.
PG  - 30579-91
LID - 10.18632/oncotarget.5769 [doi]
AB  - Cardiomyocyte apoptosis plays a causal role in the development and progression of 
      heart failure. Currently, there is no effective imaging agent that can be used to 
      detect cardiomyocyte apoptosis in vivo. To target phosphatidylserine (PS) on the 
      surface of the dying cell, we synthesized a novel 18F-labeled Zn2+-dipicolylamine 
      (DPA) analog, [18F]FP-DPAZn2, and evaluated it for noninvasive imaging of 
      cardiomyocyte apoptosis. In vitro, the fluorescence imaging of dansyl-DPAZn2 was 
      suitable for detecting cardiomyocyte apoptosis, which was confirmed by confocal 
      immunofluorescence imaging, terminal dUTP nick-end labeling (TUNEL) assay, and 
      western blot assay. The in vivo biodistribution showed that the uptake ratios of 
      [18F]FP-DPAZn2 in the heart were 4.41+/-0.29% ID/g at 5 min, 2.40 +/- 0.43% ID/g at 
      30 min, 1.63 +/- 0.26% ID/g at 60 min, and 1.43% +/- 0.07 ID/g at 120 min 
      post-injection. In vivo, the [18F]FP-DPAZn2 PET images showed more cardiac 
      accumulation of radioactivity 60 min post-injection in acute myocardial 
      infarction (AMI) rats than in normal rats, which was consistent with the findings 
      of a histological analysis of the rat cardiac tissues in vitro. [18F]FP-DPAZn2 
      PET imaging has the capability for myocardial apoptosis detection, but the method 
      will require improved myocardial uptake for the noninvasive evaluation of 
      cardiomyocyte apoptosis in clinical settings.
FAU - Sun, Ting
AU  - Sun T
AD  - Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai JiaoTong 
      University School of Medicine, Shanghai, P.R. China.
FAU - Tang, Ganghua
AU  - Tang G
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen 
      University, Guangzhou, P.R. China.
FAU - Tian, Hua
AU  - Tian H
AD  - State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute 
      and Cancer Institute of Shanghai Jiao Tong University, Shanghai, P.R. China.
FAU - Hu, Kongzhen
AU  - Hu K
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen 
      University, Guangzhou, P.R. China.
FAU - Yao, Shaobo
AU  - Yao S
AD  - Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen 
      University, Guangzhou, P.R. China.
FAU - Su, Yifan
AU  - Su Y
AD  - Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai JiaoTong 
      University School of Medicine, Shanghai, P.R. China.
FAU - Wang, Changqian
AU  - Wang C
AD  - Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai JiaoTong 
      University School of Medicine, Shanghai, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Fluorine Radioisotopes)
RN  - 0 (Molecular Probes)
RN  - 0 (Organometallic Compounds)
RN  - 0 (Picolines)
RN  - 0 (zinc(II) dipicolylamine)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cardiovascular Diseases/*diagnosis
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Echocardiography
MH  - Fluorine Radioisotopes
MH  - Heart Failure/pathology
MH  - Heart Function Tests
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Molecular Probes
MH  - Myocytes, Cardiac/*physiology
MH  - Organometallic Compounds/chemistry/*pharmacokinetics
MH  - Picolines/chemistry/*pharmacokinetics
MH  - Positron-Emission Tomography/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC4741553
OTO - NOTNLM
OT  - Pathology Section
OT  - Zinc(II)-dipicolylamine
OT  - apoptosis
OT  - cardiomyocyte
OT  - positron emission tomography imaging
OT  - radiosynthesis
COIS- CONFLICTS OF INTEREST There is no conflict of interest.
EDAT- 2015/09/30 06:00
MHDA- 2016/08/16 06:00
PMCR- 2015/10/13
CRDT- 2015/09/30 06:00
PHST- 2015/08/08 00:00 [received]
PHST- 2015/09/12 00:00 [accepted]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PHST- 2015/10/13 00:00 [pmc-release]
AID - 5769 [pii]
AID - 10.18632/oncotarget.5769 [doi]
PST - ppublish
SO  - Oncotarget. 2015 Oct 13;6(31):30579-91. doi: 10.18632/oncotarget.5769.