PMID- 26416711
OWN - NLM
STAT- MEDLINE
DCOM- 20161025
LR  - 20220330
IS  - 1534-4681 (Electronic)
IS  - 1068-9265 (Linking)
VI  - 23
IP  - 2
DP  - 2016 Feb
TI  - The Prognostic Significance of Histone Lysine Demethylase JMJD3/KDM6B in 
      Colorectal Cancer.
PG  - 678-85
LID - 10.1245/s10434-015-4879-3 [doi]
AB  - BACKGROUND: Jumonji-domain containing 3 (JMJD3) affects transcriptional 
      regulation by demethylating lysine 27 residue of histone 3. We investigated its 
      function and prognostic significance in colorectal cancer (CRC). METHODS: The 
      influence of JMJD3 on cell proliferation was assessed using quantitative RT-PCR 
      and western blot on the downstream target gene of JMJD3, in knock-down (KD) 
      experiments and clinical samples from 151 CRC patients. RESULTS: Cells with KD 
      JMJD3 significantly increased proliferation through cell cycle progression and 
      apoptosis suppression. Expression of P15INK4B was remarkably decreased in KD 
      JMJD3 cells; and JMJD3 expression strongly correlated with p15INK4B expression in 
      clinical CRC samples (P < 0.001, r = 0.566). Low JMJD3 also was an independent 
      predictor of poor prognosis (P = 0.042) in surgically resected CRC patients. 
      CONCLUSIONS: JMJD3 has prognostic significance in CRC and mediates p15INK4B 
      expression. These results imply that elucidation of the JMJD3 role may lead to a 
      new therapeutic approach for CRC patients.
FAU - Tokunaga, Ryuma
AU  - Tokunaga R
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Sakamoto, Yasuo
AU  - Sakamoto Y
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Nakagawa, Shigeki
AU  - Nakagawa S
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Miyake, Keisuke
AU  - Miyake K
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Izumi, Daisuke
AU  - Izumi D
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Kosumi, Keisuke
AU  - Kosumi K
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Taki, Katsunobu
AU  - Taki K
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Higashi, Takaaki
AU  - Higashi T
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Imamura, Yu
AU  - Imamura Y
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Ishimoto, Takatsugu
AU  - Ishimoto T
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Iwatsuki, Masaaki
AU  - Iwatsuki M
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Baba, Yoshifumi
AU  - Baba Y
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Miyamoto, Yuji
AU  - Miyamoto Y
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Yoshida, Naoya
AU  - Yoshida N
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan.
FAU - Oki, Eiji
AU  - Oki E
AD  - Department of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka, Japan.
FAU - Watanabe, Masayuki
AU  - Watanabe M
AD  - Department of Gastroenterological Surgery, Japanese Foundation for Cancer 
      Research, Tokyo, Japan.
FAU - Baba, Hideo
AU  - Baba H
AD  - Department of Gastroenterological Surgery, Graduate School of Medical Sciences, 
      Kumamoto University, Kumamoto, Japan. hdobaba@kumamoto-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20150928
PL  - United States
TA  - Ann Surg Oncol
JT  - Annals of surgical oncology
JID - 9420840
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
RN  - EC 1.14.11.- (Jumonji Domain-Containing Histone Demethylases)
RN  - EC 1.14.11.- (KDM6B protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Cycle
MH  - Cell Proliferation
MH  - Colorectal Neoplasms/enzymology/genetics/*pathology
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Jumonji Domain-Containing Histone Demethylases/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Prognosis
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Retrospective Studies
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Survival Rate
MH  - Tumor Cells, Cultured
EDAT- 2015/09/30 06:00
MHDA- 2016/10/26 06:00
CRDT- 2015/09/30 06:00
PHST- 2015/01/14 00:00 [received]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2016/10/26 06:00 [medline]
AID - 10.1245/s10434-015-4879-3 [pii]
AID - 10.1245/s10434-015-4879-3 [doi]
PST - ppublish
SO  - Ann Surg Oncol. 2016 Feb;23(2):678-85. doi: 10.1245/s10434-015-4879-3. Epub 2015 
      Sep 28.